Cloning of human acetyl-CoA carboxylase-beta and its unique features
Cloning of human acetyl-CoA carboxylase-beta and its unique features
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DOI：
10.1073/pnas.93.21.11466
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发表时间：
1996-10-15
影响因子：
11.1
通讯作者：
Kim, KH
中科院分区：
文献类型：
--
作者：
Ha, J;Lee, JK;Kim, KH
乙酰辅酶a羧化酶分子量为265 kDa (acc - α)，催化长链脂肪酸生物合成的限速步骤。在这项研究中，我们报道了ACC分子质量为275 kDa （ACC- β）的完整氨基酸序列和独特特征，该亚型主要在心脏和骨骼肌中表达。在这些组织中，acc - β可能参与脂肪酸氧化的调节，而不是脂肪酸的生物合成。acc - β在N端含有一个约200个氨基酸长的氨基酸序列，可能与acc - β在控制肉毒碱棕榈酰基转移酶I活性和线粒体脂肪酸氧化中的作用有独特的关系。如果我们排除这个独特的序列在N端，这两种形式的ACC显示约75%的氨基酸同源性。ACC的所有已知功能域都位于同源区域。人类acc - β cDNA具有7343个碱基的开放阅读框，编码2458个氨基酸的蛋白质，计算分子质量为276638 Da。人类acc - β mRNA的大小约为10 kb，主要表达于心脏和骨骼肌组织，而acc - α mRNA在所有被测组织中均可检测到。在大肠杆菌中表达了一段acc - β cDNA片段，并产生了针对该肽的抗体，证实我们克隆的cDNA序列是acc - β的cDNA序列。
Acetyl-CoA carboxylase, which has a molecular mass of 265 kDa (ACC-alpha), catalyzes the rate-limiting step in the biosynthesis of long-chain fatty acids. In this study we report the complete amino acid sequence and unique features of an isoform of ACC with a molecular mass of 275 kDa (ACC-beta), which is primarily expressed in heart and skeletal muscles. In these tissues, ACC-beta may be involved in the regulation of fatty acid oxidation, rather than fatty acid biosynthesis. ACC-beta contains an amino acid sequence at the N terminus which is about 200 amino acids long and may be uniquely related to the role of ACC-beta in controlling carnitine palmitoyltransferase I activity and fatty acid oxidation by mitochondria. If we exclude this unique sequence at the N terminus the two forms of ACC show about 75% amino acid identity. All of the known functional domains of ACC are found in the homologous regions. Human ACC-beta cDNA has an open reading frame of 7,343 bases, encoding a protein of 2,458 amino acids, with a calculated molecular mass of 276,638 Da. The mRNA size of human ACC-beta is approximately 10 kb and is primarily expressed in heart and skeletal muscle tissues, whereas ACC-alpha mRNA is detected in all tissues tested. A fragment of ACC-beta cDNA was expressed in Escherichia coli and antibodies against the peptide were generated to establish that the cDNA sequence that we cloned is that for ACC-beta.