Research note: effects of age and immunization interval on the anamnestic response to T-cell-dependent and T-cell-independent antigens in chickens.
Research note: effects of age and immunization interval on the anamnestic response to T-cell-dependent and T-cell-independent antigens in chickens.
复制标题
研究报告：年龄和免疫间隔对鸡对 T 细胞依赖性和 T 细胞非依赖性抗原的记忆反应的影响。
DOI：
10.3382/ps.0702371
复制
发表时间：
1991
期刊：
影响因子：
4.4
通讯作者：
S. Lamont
中科院分区：
文献类型：
--
作者：
P. L. Munns;S. Lamont
体液记忆反应的测量是用于评估免疫能力的常用程序。本研究的目的是确定获得最大抗体效价所需的初次免疫和二次免疫之间的最佳时间段，并确定该间隔是否因鸡龄或诱导抗原的类型而异。评估了两个年龄的鸟类对 T 细胞依赖性抗原 (SRBC) 和 T 细胞非依赖性抗原 (流产布鲁氏菌，BA) 的记忆反应。对 4 周或 6 个月龄的鸡进行静脉注射。使用 BA 或 SRBC，并在 2、4、6 或 8 周后进行二次注射。从二次免疫后 3、6 和 9 天收集的血清样品中测定总抗体和巯基乙醇 (ME) 抗性抗体的凝集滴度。一般来说，二次免疫后 6 天滴度最高。对于这两种抗原，二次免疫后第 6 天的雏鸡总抗体水平在 4、6 和 8 周免疫间隔时均高于 2 周免疫间隔；成人滴度与免疫间隔无关。鸟类的年龄和抗原类型之间存在相互作用。
Measurement of a humoral anamnestic response is a common procedure used to evaluate immunological competence. The purposes of the present study were to determine the optimum time period between primary and secondary immunizations needed to obtain the maximum antibody titer and to determine whether this interval differs with the age of the chicken or with the type of inducing antigen. The anamnestic responses of birds of two ages to a T-cell-dependent antigen (SRBC) and to a T-cell-independent antigen (Brucella abortus, BA) were evaluated. Birds of 4 wk or 6 mo of age were injected i.v. with either BA or SRBC, and a secondary injection was given 2, 4, 6, or 8 wk later. Agglutination titers for total and mercaptoethanol (ME)-resistant antibodies were determined from serum samples collected at 3, 6, and 9 days postsecondary immunization. Generally, titers were highest at 6 days postsecondary immunization. For both antigens, the chick total antibody levels at Day 6 postsecondary immunization were higher at 4-, 6-, and 8-wk immunization intervals than at 2-wk intervals; adult titers were independent of immunization interval. There was an interaction between age of birds and type of antigen.