Production and characterization of intergeneric hybrids between Brassica oleracea and a wild relative Moricandia arvensis
Production and characterization of intergeneric hybrids between Brassica oleracea and a wild relative Moricandia arvensis
复制标题
DOI：
10.1111/j.1439-0523.2007.01307.x
复制
发表时间：
2007-02-01
期刊：
影响因子：
2
通讯作者：
Matsuzawa, Y.
中科院分区：
文献类型：
--
作者：
Bang, S. W.;Sugihara, K.;Matsuzawa, Y.
利用胚拯救技术进行甘蓝和田Moricandia arvensis的属间杂交。以B为母本进行杂交，获得6个杂种F1代植株。oleracea x M.通过胎盘胚培养技术从64个荚中产生了Arvensis，而通过子房培养技术从40个荚中正反交产生了3个植株。杂种植株为双单倍体，有丝分裂染色体数为2n = 23，减数分裂中期Ⅰ（M Ⅰ）染色体组合为（0-3）Ⅱ +（17-23）Ⅰ。在与B的背交中。在甘蓝中，部分杂种获得了染色体数为2n = 32的倍半二倍体BC 1植株，花粉母细胞减数分裂构型主要为（9 Ⅱ +14 Ⅰ）。将BC 2植物与B回交。甘蓝BC_3代共产生48个体细胞染色体数目在2n = 19 ~ 41之间的后代。2n = 19株的染色体组合类型为（9 Ⅱ +1 Ⅰ），M Ⅰ和M Ⅱ的染色体分布类型分别为（9（1）/（2）+ 9（1）/（2））和（9 + 10）。这些事实可能表明它们是B的单体附加系。oleracea携带M. arvensis，可以提供潜在的未来的遗传和育种研究，以及其他新的杂交后代在这种属间杂交。
Intergeneric crosses were made between Brassica oleracea and Moricandia arvensis utilizing embryo rescue. Six F-1 hybrid plants were generated in the cross-combination of B. oleracea x M. arvensis from 64 pods by the placenta-embryo culture technique, whereas three plants were produced in the reciprocal cross from 40 pods by the ovary culture technique. The hybrid plants were ascertained to be amphihaploid with 2n = 23 chromosomes in mitosis and a meiotic chromosome association of (0-3)II + (17-23)I at metaphase I (M I). In the backcross with B. oleracea, some of these hybrids developed sesquidiploid BC1 plants with 2n = 32 chromosomes that predominantly exhibited a meiotic configuration of (9II + 14I) in pollen mother cells. The following backcross of BC2 plants to B. oleracea generated 48 BC3 progeny with somatic chromosomes from 2n = 19 to 2n = 41. The 2n = 19 plants showed a chromosomal association type of (9II + 1I) and a chromosomal distribution type of (9(1)/(2) + 9(1)/(2)) or (9 + 10) at M I and M II, respectively. These facts might suggest that they were monosomic addition lines (MALs) of B. oleracea carrying a single chromosome of M. arvensis that could offer potential for future genetic and breeding research, together with other novel hybrid progeny developed in this intergeneric hybridization.