Development of in vitro gene delivery system using ORMOSIL nanoparticle: Analysis of p53 gene expression in cultured breast cancer cell (MCF-7)
Development of in vitro gene delivery system using ORMOSIL nanoparticle: Analysis of p53 gene expression in cultured breast cancer cell (MCF-7)
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使用 ORMOSIL 纳米颗粒开发体外基因传递系统：培养乳腺癌细胞 (MCF-7) 中 p53 基因表达的分析
DOI：
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发表时间：
2012
影响因子：
5.7
通讯作者：
K. Muthuchelian
中科院分区：
文献类型：
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作者：
C. Rejeeth;S. Kannan;K. Muthuchelian
本文综述了近年来有机修饰二氧化硅纳米粒作为一种高效的体外基因传递系统的应用。基于这一主要目标，本研究探讨了在细胞水平上降低癌症发病率的可能途径。在此背景下，合成了ORMOSIL纳米颗粒，并与携带p53基因的pCMV-Myc（3.8 kb）质粒载体构建体一起沿着孵育，并转染到乳腺癌细胞系MCF-7细胞中。Western blot分析显示p53蛋白在乳腺癌细胞中有明显表达。共聚焦显微镜和电子显微镜研究进一步证实，纳米颗粒积聚在转染p53基因的癌细胞的细胞质和细胞核中。有趣的琼脂糖凝胶电泳研究表明，纳米颗粒有效地与pCMV-Myc载体复合。p53的抗癌特性通过评估细胞存活率和生长速率来证明，其在癌细胞中显示出正线性相关。然而，与未转染细胞的生长速率相比，ORMOSIL/p53/pCMV-Myc转染的乳腺癌细胞的生长速率显著降低。使用ORMOSIL纳米颗粒作为非病毒基因递送平台的这种方法的结果具有作为有效的转染剂用于癌细胞的治疗操作和体内靶向癌症基因治疗的前景。
This article reports on the application of organically modified silica (ORMOSIL) nanoparticles as an efficient in vitro gene delivery system in the recent years. Based on that prime objective, the present study addresses the possible ways to reduce cancers incidence at cellular level. In this context, ORMOSIL nanoparticles had been synthesized and incubated along with pCMV–Myc (3.8 kb) plasmid vector construct carrying p53gene, and transfected into the breast cancer cell line MCF-7 cells. Western blot analysis showed that the p53 protein was significantly expressed in breast cancer cell upon transfection. The confocal and electron microscopic studies further confirmed that the nanoparticles were accumulated in the cytoplasm and the nucleus of the cancer cells transfected with p53 gene. Interesting agarose gel electrophoresis studies revealed that the nanoparticles efficiently complex with pCMV–Myc vector. The anti-cancer properties of p53 were demonstrated by assessing the cell survival and growth rate which showed a positive linear correlation in cancer cells. Whereas, the growth rate was significantly reduced in ORMOSIL/p53/pCMV–Myc transfected breast cancer cells compared to the growth rate of non-transfected cells. The results of this approach using ORMOSIL nanoparticles as a non-viral gene delivery platform have a promising future for use as effective transfection agent for therapeutic manipulation of cancer cells and targeted cancer gene therapy in vivo.
影响因子：
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作者：
Kumar, MNVR;Sameti, M;Lehr, CM
通讯作者：
Lehr, CM