Identification and kinetic study of tyrosinase inhibitors found in sake lees
Identification and kinetic study of tyrosinase inhibitors found in sake lees
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DOI：
10.1021/jf062315p
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发表时间：
2006-12-27
影响因子：
6.1
通讯作者：
Sugiyama, Masanori
中科院分区：
文献类型：
--
作者：
Jeon, Hyung Joon;Noda, Masafumi;Sugiyama, Masanori
本研究发现，冷冻干燥酒糟的正己烷提取物对酪氨酸酶活性有抑制作用，并表明从正己烷提取物中分离出的成分是三酰甘油的混合物。用蘑菇酪氨酸酶和卡氏链霉菌酪氨酸酶对三酸甘油酯和三甘油酯的抑制作用进行了研究。三酰甘油混合物对蘑菇酪氨酸酶和链霉菌酪氨酸酶的IC50值分别为20和0.14 mU g/m L。三甘油酯对蘑菇酪氨酸酶和链霉菌酪氨酸酶的IC50值分别为8.4和0.1mU·M。然而，即使用酪氨酸链霉菌进行测定，三油酸甘油酯(IC50=30 mU M)的抑制作用也比三油酸甘油酯低。动力学分析表明，三酸甘油酯和三酸甘油酯均非竞争性抑制酪氨酸酶活性。当转化链霉菌酪氨酸酶基因时，转化的大肠杆菌宿主的黑色素合成能力受到三甘油酯的剂量依赖性干扰。
The present study found that the n-hexane extract of freeze-dried sake lees inhibits tyrosinase activity and showed that the constituents isolated from the n-hexane extract are the mixture of triacylglycerols. The inhibitory effects of triolein and trilinolein found as the triacylglycerols were examined using tyrosinases from mushroom and Streptomyces castaneoglobisporus. The IC50 values of the triacylglycerol mixture for the oxidase activity on mushroom and Streptomyces tyrosinases were 20 and 0.14 mu g/mL, respectively. The IC50 values of trilinolein for the oxidase activity on mushroom and Streptomyces tyrosinases were 8.4 and 0.1 mu M, respectively. However, the inhibitory effect of triolein (IC50 = 30 mu M) was lower than that of trilinolein, even when the Streptomyces tyrosinase was used for the assay. Kinetic analyses indicate that both trilinolein and triolein inhibit the tyrosinase activity noncompetitively. When transformed with a plasmid carrying the Streptomyces tyrosinase gene, the melanin-synthesizing ability of the transformed Escherichia coli host was dose-dependently interfered with by trilinolein.