Promoting effect of amino acids added to a chemically defined medium on blastocyst formation and blastomere proliferation of bovine embryos cultured in vitro
Promoting effect of amino acids added to a chemically defined medium on blastocyst formation and blastomere proliferation of bovine embryos cultured in vitro
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DOI：
10.1016/j.anireprosci.2004.02.003
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发表时间：
2004-09-01
影响因子：
2.2
通讯作者：
Hwang, WS
中科院分区：
文献类型：
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作者：
Lee, ES;Fukui, Y;Hwang, WS
本研究旨在阐明氨基酸单独或成组添加到化学定义的培养基中对牛胚囊胚形成和卵裂球增殖的作用。通过体外受精获得胚胎，形成囊胚并孵化，胚胎在人工输卵管液培养基（SOFM）中培养后，监测囊胚的卵裂球数量。首先，在SOFM中添加四种非必需氨基酸（天冬酰胺、天冬氨酸、谷氨酸或丝氨酸）中的一种，与不添加相比，添加天冬酰胺、天冬氨酸或谷氨酸后囊胚形成显著（P < 0.05）增加（35-42%对22%）。其次，添加四种必需氨基酸（精氨酸、胱氨酸、异亮氨酸或亮氨酸）中的一种，精氨酸或异亮氨酸大大改善了囊胚的形成（30-36%对16%）。第三，在SOFM中添加5种刺激氨基酸（天冬氨酸、天冬氨酸、谷氨酸、精氨酸和异亮氨酸）与不添加相比，显著改善了囊胚形成（12%比21%），这一值与添加由MEM氨基酸溶液组成的19种氨基酸后的结果相似（21-27%）。然而，5种氨基酸产生的囊胚孵化量少于19种氨基酸。最后，虽然添加5种氨基酸的囊胚细胞数比不添加的囊胚细胞数多（93个比74个），但低于添加19种氨基酸的囊胚细胞数（131个）。综上所述，天冬氨酸、天冬氨酸、谷氨酸、精氨酸和异亮氨酸的单独或联合添加均可促进囊胚的形成，而其他氨基酸可能是进一步刺激囊胚增殖和囊胚孵化所必需的。(C) 2004 Elsevier B.V.版权所有
This study was conducted to elucidate the role of amino acids added singly or in groups to a chemically defined culture medium in blastocyst formation and blastomere proliferation of bovine embryos. Embryos were generated by in vitro fertilization, and blastocyst formation and hatching, and blastomere number of blastocysts were subsequently monitored after the culture of embryos in synthetic oviduct fluid medium (SOFM). First, one of four non-essential amino acids (asparagine, aspartate, glutamate or serine) was added to SOFM and, compared with no addition, a significant (P < 0.05) increase in blastocyst formation was found after the addition of asparagine, aspartate, or glutamate (35-42% versus 22%). Second, one of four essential amino acids (arginine, cystine, isoleucine or leucine) was added and arginine or isoleucine greatly improved blastocyst formation (30-36% versus 16%). Third, the addition of five stimulatory amino acids (aspartate, asparagine, glutamate, arginine and isoleucine) to SOFM significantly improved blastocyst formation compared with no addition (12% versus 21%) and such value was similar to that obtained after the addition of 19 amino acids consisting of MEM amino acid solutions (21-27%). However, five amino acids yielded fewer hatched blastocysts than 19 amino acids. Finally, although five amino acids yielded more cell number of blastocysts than no addition (93 versus 74 cells per blastocyst), it was lower than that from 19 amino acids (131 cells per blastocyst). In conclusion, either single or combined addition of asparagine, aspartate, glutamate, arginine and isoleucine stimulated blastocyst formation, while other amino acids might be necessary for further stimulating blastomere proliferation and blastocyst hatching. (C) 2004 Elsevier B.V. All rights reserved.