TRANSPLANTATION OF CARTILAGENOUS TISSUE GENERATED IN-VITRO INTO ARTICULAR JOINT DEFECTS
TRANSPLANTATION OF CARTILAGENOUS TISSUE GENERATED IN-VITRO INTO ARTICULAR JOINT DEFECTS
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DOI：
10.3109/10731199509117971
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发表时间：
1995-01-01
影响因子：
--
通讯作者：
RENLUND, R
中科院分区：
文献类型：
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作者：
KANDEL, RA;CHEN, H;RENLUND, R
这项初步研究的目的是确定分离的兔软骨细胞是否会在培养中形成软骨组织，以及这种组织是否可以用作软骨移植物，以修复受损的关节。从兔关节软骨中分离软骨细胞，将其作为单层铺在Millicell-CM（R）过滤器上并维持在细胞培养物中。细胞形成软骨组织，可以在培养两周后从滤器支架上去除。软骨细胞合成II型胶原，表明它们在这些条件下保持其表型。对于移植研究，在家兔中创建了两种类型的关节面缺损，即软骨下骨全层缺损或软骨内缺损。将软骨组织放置在缺损处，不固定或局部应用粘合剂（Cell-Tak或Nexaband（R）Avian）。在手术后两周内检查关节。在尝试用Cell-Tak进行组织固定的动物中，没有移植物留在缺损处。用Nexaband（R）Avian固定到软骨缺损中的那些移植物保持在原位，但由一层浓缩的无细胞组织组成。然而，在没有粘合剂的情况下，将移植物植入全层缺损的6只动物中，有5只动物的软骨组织存在且完整。总之，体外生成的软骨组织可以在移植后存活，但需要适当的方法将移植物固定到软骨内缺损中。
The purpose of this pilot study was to determine whether isolated rabbit chondrocytes will form cartilagenous tissue in culture and whether this tissue can be used as a cartilage transplant in order to resurface damaged joints. Chondrocytes were isolated from rabbit articular cartilage, plated as a monolayer on Millicell-CM(R) filters and maintained in cell culture. The cells formed cartilagenous tissue that could be removed from the filter support by two weeks in culture. The chondrocytes synthesize type II collagen indicating that they maintained their phenotype under these conditions. For the transplant studies, two types of articular surface defects, either full thickness into subchondral bone or intra-chondral, were created in rabbits. The cartilagenous tissue was placed in the defect either without fixation or with the topical application of an adhesive agent (Cell-Tak or Nexaband(R) Avian). The joints were examined within two weeks following the surgery. No transplants remained in the defects in those animals in which tissue fixation had been attempted with Cell-Tak. Those grafts fixed into the cartilage defect with Nexaband(R) Avian remained in place but consisted of a condensed layer of acellular tissue. However, cartilagenous tissue was present and intact in five of the six animals in which the transplant had been placed, in the absence of adhesive, into a full thickness defect. In conclusion, cartilagenous tissue generated in vitro can survive transplantation but an appropriate method to fix grafts into intra-chondral defects is required.