Salidroside attenuates glutamate-induced apoptotic cell death in primary cultured hippocampal neurons of rats
Salidroside attenuates glutamate-induced apoptotic cell death in primary cultured hippocampal neurons of rats
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DOI：
10.1016/j.brainres.2008.07.051
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发表时间：
2008-10-31
期刊：
影响因子：
2.9
通讯作者：
Ding, Fei
中科院分区：
文献类型：
--
作者：
Chen, Xia;Liu, Jie;Ding, Fei
红景天苷是一种天然化合物，具有广泛的药理活性。本研究旨在评估红景天苷对谷氨酸诱导的大鼠海马神经元细胞死亡的抑制作用，并与脑源性神经营养因子（BDNF）进行比较。MTT法、LDH法、Hoechst 33342染色法、TUNEL法、Annexin-V和PI标记流式细胞仪检测结果表明，红景天苷预处理可明显抑制谷氨酸诱导的原代培养海马神经元凋亡，且呈剂量依赖性。此外，caspase-3活性测定和钙测量与Fluo 4-AM，分别显示，红景天苷预处理拮抗caspase-3的激活和细胞内钙水平的升高，这两个都是由谷氨酸刺激诱导的，从而增加了理解红景天苷如何提供神经保护对谷氨酸兴奋性毒性。(C)2008 Elsevier B. V.保留所有权利。
Salidroside, a compound of natural origin, has displayed a broad spectrum of pharmacological properties. This study aimed to evaluate the inhibitory effects of salidroside on glutamate-induced cell death in a primary culture of rat hippocampal neurons as compared to brain-derived neurotrophic factor (BDNF), a usual positive control. MTT and LDH assays, together with Hoechst 33342 staining, terminal deoxynucleotidyl transferase dUTP-mediated nicked end labeling (TUNEL) assay and flow cytometric analysis using annexin-V and propidium (PI) label, indicated that salidroside pretreatment attenuated glutamate-induced apoptotic cell death in primary cultured hippocampal neurons, showing a dose-dependent pattern. Furthermore, caspase-3 activity assay and calcium measurements with Fluo 4-AM, respectively, revealed that salidroside pretreatment antagonized activation of caspase-3 and elevation of intracellular calcium level, both of which were induced by glutamate stimulation, thus adding to the understanding of how salidroside offered neuroprotection against glutamate excitotoxicity. (C) 2008 Elsevier B.V. All rights reserved.