Reducing, radical scavenging, and chelation properties of in vitro digests of alcalase-treated zein hydrolysate
Reducing, radical scavenging, and chelation properties of in vitro digests of alcalase-treated zein hydrolysate
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DOI：
10.1021/jf703697e
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发表时间：
2008-04-23
影响因子：
6.1
通讯作者：
Xiong, Youling L.
中科院分区：
文献类型：
--
作者：
Zhu, Lijuan;Chen, Jie;Xiong, Youling L.
本研究的目的是评估碱性酶处理的玉米醇溶蛋白水解物(ZH)在体外两个阶段(胃酶1h-GT；胰酶0.5h-2h，37℃)的抗氧化潜力。采用Sephadex凝胶过滤和高效体积排阻色谱分离得到ZH组分。氨基酸组成，2，2‘-azinobis(3-ethylbenzothiazoline-6-sulfonic酸)(Abts(+.))和1，1-二苯基-2-苦基肼(DPPH.)测定了ZH的自由基清除能力、还原能力和Cu2+的络合能力，以确定ZH的抗氧化效果。结果表明，ZH体外消化液中游离氨基酸含量高达16.5%，且含有短肽(
The objective of the study was to assess the antioxidant potential of alcalase-treated zein hydrolysate (ZH) during a two-stage (1 h of pepsin --> 0.5-2 h of pancreatin, 37 degrees C) in vitro digestion. Sephadex gel filtration and high-performance size exclusion chromatography were used to separate ZH into fractions. The amino acid composition, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS(+.)) and 1, 1-diphenyl-2-picrylhydrazyl (DPPH.) free radical scavenging activity, reducing power, and CU2+ chelation ability were tested to determine the antioxidant efficacy of ZH. Results showed that in vitro digests of ZH contained up to 16.5% free amino acids, with short peptides (