Screening of Compounds against Gardnerella vaginalis Biofilms.
Screening of Compounds against Gardnerella vaginalis Biofilms.
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DOI：
10.1371/journal.pone.0154086
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Wagner-Döbler I
中科院分区：
文献类型：
--
作者：
Gottschick C;Szafranski SP;Kunze B;Sztajer H;Masur C;Abels C;Wagner-Döbler I
细菌性阴道病（细菌性阴道病）是育龄妇女的一种常见感染，其特点是以乳酸菌为主的健康阴道菌群失调，其次是阴道加德纳菌等细菌的生长。阴道支原体形成生物膜的能力导致了细菌性阴道炎的高复发率，这是典型的细菌性阴道炎，不幸的是，反复的抗生素治疗是不可避免的。在此，我们建立了阴道弧菌的生物膜模型，并筛选了大量化合物，以了解它们阻止生物膜形成和分解现有阴道弧菌生物膜的能力。抗生素甲硝唑和妥布霉素对防止生物膜的形成非常有效，但对已形成的生物膜没有影响。两性张力素椰两性醋酸钠（SCAA）的使用导致现有生物膜的崩解，使生物量降低51%，活力降低61%，使甲硝唑的效果提高40%（生物量），61%（活力）。我们的数据表明，通过两性张力蛋白与抗生素甲硝唑的结合来攻击生物膜和细菌细胞可能是一种有效的对抗BV的策略。
Bacterial vaginosis (BV) is a common infection in reproductive age woman and is characterized by dysbiosis of the healthy vaginal flora which is dominated by Lactobacilli, followed by growth of bacteria like Gardnerella vaginalis. The ability of G. vaginalis to form biofilms contributes to the high rates of recurrence that are typical for BV and which unfortunately make repeated antibiotic therapy inevitable. Here we developed a biofilm model for G. vaginalis and screened a large spectrum of compounds for their ability to prevent biofilm formation and to resolve an existing G. vaginalis biofilm. The antibiotics metronidazole and tobramycin were highly effective in preventing biofilm formation, but had no effect on an established biofilm. The application of the amphoteric tenside sodium cocoamphoacetate (SCAA) led to disintegration of existing biofilms, reducing biomass by 51% and viability by 61% and it was able to increase the effect of metronidazole by 40% (biomass) and 61% (viability). Our data show that attacking the biofilm and the bacterial cells by the combination of an amphoteric tenside with the antibiotic metronidazole might be a useful strategy against BV.