Each of the eight simian hemorrhagic fever virus minor structural proteins is functionally important.
Each of the eight simian hemorrhagic fever virus minor structural proteins is functionally important.
复制标题
八种猿猴出血热病毒中的每一个在功能上很重要。
DOI：
10.1016/j.virol.2014.06.001
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发表时间：
2014-08
期刊：
影响因子：
3.7
通讯作者：
Brinton, Margo A.
中科院分区：
文献类型：
--
作者：
Vatter, Heather A.;Di, Han;Donaldson, Eric F.;Baric, Ralph S.;Brinton, Margo A.
猴出血热病毒（SHFV）基因组与动脉炎病毒科其他成员的基因组不同，它编码两组相邻的四个次要结构蛋白开放阅读框（ORF）。构建了一个稳定的、全长的、具有感染性的SHFV - LVR cDNA克隆。由该克隆产生的病毒具有与亲本病毒相似的复制特征。针对先前被鉴定为2b的SHFV ORF，鉴定出了一种亚基因组mRNA。作为分析每种SHFV次要结构蛋白功能相关性的初步手段，构建了一组突变的感染性克隆，每个克隆中一个次要结构蛋白ORF的起始密码子发生突变。对于成对的次要糖蛋白的每个直系同源物观察到不同的表型，并且所有八种次要结构蛋白都是产生具有感染性的细胞外病毒所必需的，这表明SHFV次要结构蛋白的重复组在功能上不是冗余的。
The simian hemorrhagic fever virus (SHFV) genome differs from those of other members of the family Arterivirus in encoding two adjacent sets of four minor structural protein open reading frames (ORFs). A stable, full-length, infectious SHFV-LVR cDNA clone was constructed. Virus produced from this clone had replication characteristics similar to those of the parental virus. A subgenomic mRNA was identified for the SHFV ORF previously identified as 2b. As an initial means of analyzing the functional relevance of each of the SHFV minor structural proteins, a set of mutant infectious clones was generated, each with the start codon of one minor structural protein ORF mutated. Different phenotypes were observed for each ortholog of the pairs of minor glycoproteins and all of the eight minor structural proteins were required for the production of infectious extracellular virus indicating that the duplicated sets of SHFV minor structural proteins are not functionally redundant.
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影响因子：
3.7
作者：
Lee C;Yoo D
通讯作者：
Yoo D
影响因子：
2.7
作者：
Dea S;Gagnon CA;Mardassi H;Pirzadeh B;Rogan D
通讯作者：
Rogan D
DOI：
10.4269/ajtmh.1968.17.404
发表时间：
1968-01-01
影响因子：
3.3
作者：
PALMER, AE;ALLEN, AM;SHELOKOV, A
通讯作者：
SHELOKOV, A
影响因子：
5.4
作者：
Delputte, Peter L.;Van Breedam, Wander;Nauwynck, Hans J.
通讯作者：
Nauwynck, Hans J.
影响因子：
5.4
作者：
Delputte, PL;Vanderheijden, N;Pensaert, MB
通讯作者：
Pensaert, MB