Temperature-dependent disposition of [14C]benzo(a)pyrene in the spiny lobster, Panulirus argus.
Temperature-dependent disposition of [14C]benzo(a)pyrene in the spiny lobster, Panulirus argus.
复制标题
大龙虾（Panulirus argus）中[14C]苯并（a）芘的温度依赖性处置。
DOI：
10.1016/0041-008x(85)90332-1
复制
发表时间：
1985
影响因子：
3.8
通讯作者：
J. Bend
中科院分区：
文献类型：
--
作者：
Peter J. Little;M. O. James;M. O. James;John B. Pritchard;J. Bend
[14 C]苯并芘（BP）（1 mg/kg）通过心内注射给予多刺龙虾组，在给药后7周内的不同时间处死。评价组织和体液的BP衍生放射性。在连续的夏季和冬季进行了两项研究，当时海水温度分别为26.5至29.0和13.5至16.5°C。在肝胰腺、胃、肠、肠内容物和绿色腺中发现最高浓度的BP衍生放射性。在初始分布阶段之后，剂量以对数线性方式从龙虾中损失。在较暖（夏季）水中，BP衍生放射性总体消除的消除半衰期为1.11周，在较冷（冬季）水中为2.25周。同样，对于单个器官，在较温暖的水中消除更快。对于肝胰腺、绿色腺、肠道和尾肌，夏季的t1 2值（周）分别为1.02、1.26、1.71和1.42，冬季分别为2.50、1.50、5.04和2.11。未提示BP衍生放射性的组织蓄积。肝胰腺样品的HPLC分析表明，在夏季，未代谢的BP浓度迅速下降，仅占5%的总标签在肝胰腺的3天。非代谢BP的下降伴随着极性代谢物和共轭物百分比的大致相等的增加。虽然在冬季没有研究BP在肝胰腺中代谢的时间曲线，但代谢能力是这样的，在给药后3天，肝胰腺中存在的14 C中只有5%是未代谢的BP。因此，对于该剂量的BP，夏季14 C的快速消除似乎是由于代谢产物的排泄更快，而不是BP代谢的增加。
[14C]Benzo(a) pyrene (BP) (1 mg/kg) was administered by intracardiac injection to groups of spiny lobsters which were killed at various times up to 7 weeks after dosing. Tissues and fluids were evaluated for BP-derived radioactivity. Two studies were conducted in successive summer and winter seasons, when seawater temperatures throughout were 26.5 to 29.0 and 13.5 to 16.5°C, respectively. Highest concentrations of BP-derived radioactivity were found in the hepatopancreas, stomach, intestine, intestinal contents, and the green gland. After an initial distribution phase, the dose was lost from the lobsters in a log linear manner. The elimination half-lives for overall elimination of BP-derived radioactivity were 1.11 weeks in the warmer (summer) and 2.25 weeks in the colder (winter) water. Similarly, for individual organs, elimination was more rapid in the warmer water. For the hepatopancreas, green gland, intestine, and tail muscle, respective t 1 2 values (week) were 1.02, 1.26, 1.71, and 1.42 in the summer and 2.50, 1.50, 5.04, and 2.11 in the winter. There was no suggestion of tissue accumulation of BP-derived radioactivity. HPLC analysis of hepatopancreas samples showed that, in summer, unmetabolized BP concentrations fell rapidly, accounting for only 5% of the total label in the hepatopancreas by 3 days. The fall in unmetabolized BP was accompanied by approximately equal increases in the percentages of both polar metabolites and conjugates. Although the time curve for metabolism of BP in the hepatopancreas was not studied in winter, the metabolic capacity was such that, by 3 days after the dose, only 5% of the14C present in hepatopancreas was unmetabolized BP. Thus, it appears that, for this dose of BP, the more rapid elimination of14C in summer was due to a more rapid excretion of metabolites, and not to increased metabolism of BP.