Standardized assessment of cell proliferation:: The approach of the RITA-CEPA working group
Standardized assessment of cell proliferation:: The approach of the RITA-CEPA working group
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DOI：
10.1016/j.etp.2005.06.002
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发表时间：
2005-11-01
影响因子：
--
通讯作者：
Weber, E
中科院分区：
文献类型：
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作者：
Nolte, T;Kaufmann, W;Weber, E
自1985年以来，细胞增殖的定量数据日益成为许多药物、化学和农用化合物风险评估的重要组成部分。进行细胞增殖研究的技术指南并不是现成的。细胞增殖的检测几乎完全依赖于免疫组织化学，而这在实验室之间很少是标准化的。在此背景下，病理学家组成了RITA-CEPA工作组，目的是为进行细胞增殖研究制定标准指南。作为例子，我们提出了大鼠肝细胞、甲状腺滤泡细胞和肾小管上皮细胞的一般BrdU指南以及器官特异性指南。今天，RITA-CEPA已经提供了20个器官中BrdU细胞增殖研究的指南，以及使用增殖细胞核抗原作为增殖标记物的研究和使用TUNEL技术进行细胞凋亡研究的指南。500多份选定出版物的相关信息被组织在一个数据库结构中，以便于追踪。正在评估用于图像分析的新硬件和软件，并将其纳入CEPA工作组进行的信息交换。此外，还研究了与细胞增殖研究重要相关的领域，如GLP环境中图像分析设备的统计和验证。正在编写一份关于在细胞增殖研究中使用统计数据的指南。CEPA小组成员定期举行会议，交流信息。他们使用电子数据库来规划和解释细胞增殖研究。CEPA将继续编写器官指南，并扩大其他增殖标记物的系列，例如Ki-67。它促进了应用于增殖研究的技术的标准化，特别是免疫组织化学和图像分析。CEPA小组的成员可以获得关于细胞增殖各个方面的全面和稳步增加的知识库。CEPA小组的成员资格对所有开发化学、农用化学品或医药产品的行业组织开放。(C)2005年爱思唯尔股份有限公司。版权所有。
Since 1985, quantitative data on cell proliferation increasingly form an integral part in the risk assessment of many pharmaceutical, chemical, and agrochemical compounds. Technical guides for the conduct of cell proliferation studies are not readily available. The detection of cell proliferation relies almost exclusively on immunohistochemistry, which is rarely standardized between laboratories. Against this background pathologists formed the RITA-CEPA working group with the aim of elaborating standard guides for the conduct of cell proliferation studies. We present here, as examples, the general BrdU guide as well as the organ-specific guides for hepatocytes, thyroid gland follicular cells, and renal tubular epithelial cells in the rat. Today, RITA-CEPA has available guides for BrdU cell proliferation studies in 20 organs as well as for studies with PCNA as proliferation marker and for apoptosis studies with the TUNEL technique. The relevant information from more than 500 selected publications is organized in a database structure to make it easily traceable. New hardware and software used for image analysis are being assessed and are included in the information exchange practiced in the CEPA working group.Further fields of major relevance for cell proliferation studies, such as statistics and the validation of image analysis equipment in a GLP environment are investigated. A guide on the use of statistics in cell proliferation studies is in preparation.CEPA-group members meet regularly to exchange information. They use the electronic database for planning and interpretation of cell proliferation studies. CEPA will continue with the preparation of organ guides and expand the series for other proliferation markers, e.g. Ki-67. It promotes standardization of techniques applied in proliferation studies, especially immunohistochemistry and image analysis. Members of the CEPA-group have access to a comprehensive and steadily increasing knowledge base on all aspects of cell proliferation. Membership of the CEPA group is open for all industrial organizations who develop chemical, agrochemical, or pharmaceutical products. (c) 2005 Elsevier GmbH. All rights reserved.