The effects of glucose concentration on the reciprocal regulation of rRNA promoters in Plasmodium falciparum
The effects of glucose concentration on the reciprocal regulation of rRNA promoters in Plasmodium falciparum
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DOI：
10.1074/jbc.m308284200
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发表时间：
2004-01-02
影响因子：
4.8
通讯作者：
McCutchan, TF
中科院分区：
文献类型：
--
作者：
Fang, J;Sullivan, M;McCutchan, TF
恶性疟原虫的发育过程对葡萄糖浓度非常敏感。我们研究了葡萄糖浓度对寄生虫发育周期的影响，通过核糖体RNA （rRNA）转录的变化来反映。我们发现，葡萄糖饥饿对rRNA基因的转录控制有不同的影响，通过急剧抑制与寄生虫无性发育有关的基因座的转录，而上调与寄生虫有性发育有关的基因座的转录。温度变化也影响转录调控。我们发现温度和葡萄糖的作用是协同的。我们鉴定并比较了每个基因转录起始位点的上游区域。这些假定的启动子结构彼此差异很大，并且包含与其他生物中的rRNA控制元件非常相似的结构。
The developmental progression of Plasmodium falciparum is remarkably sensitive to glucose concentration. We have investigated the effects of glucose concentration on the parasite development cycle as reflected by changes of ribosomal RNA ( rRNA) transcription. We showed that glucose starvation differentially affects transcriptional control of the rRNA genes by sharply repressing transcription from those loci involved with asexual development of the parasite while up-regulating transcription at those loci involved with sexual development of the parasite. Temperature change also effects regulation of transcription. We found that the effects of temperature and glucose were synergistic. We identified and compared the upstream region of the transcription start sites of each gene. These putative promoter structures are considerably different from one another and contain structures remarkably similar to rRNA control elements in other organisms.