Universal Methods for Transgene Induction Using the Dexamethasone-Inducible Transcription Activation System pOp6/LhGR in Arabidopsis and Other Plant Species.
Universal Methods for Transgene Induction Using the Dexamethasone-Inducible Transcription Activation System pOp6/LhGR in Arabidopsis and Other Plant Species.
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DOI：
10.1002/cppb.20089
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发表时间：
2019-06-01
影响因子：
--
通讯作者：
Moore, Ian
中科院分区：
文献类型：
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作者：
Samalova, Marketa;Kirchhelle, Charlotte;Moore, Ian
使用化学诱导系统进行转基因表达是现代植物生物学研究的关键要求，因为它允许(1)在组成性表达时表达损害植物活力或肥力的转基因；(2)对转基因表达水平的时空控制。我们描述了严格调控和高响应的地塞米松诱导的基因表达系统pOp6/LhGR，它包括嵌合转录激活子LhGR和相应的pOp6启动子。诱导后，LhGR激活子与pOp6启动子结合，诱导目标基因的表达。我们提供了在不同发育阶段和不同植物物种中诱导转基因表达的详细方案，并讨论了地塞米松的稳定性及其类似物的使用。我们还介绍了新的、通用的、网关兼容的二进制矢量，现在可用于pOp6/LhGR系统。©2019 by John Wiley & Sons, Inc。
Use of chemically inducible systems for transgene expression is a crucial requirement for modern plant biology research, as it allows (1) expression of transgenes that compromise plant viability or fertility when constitutively expressed and (2) spatiotemporal control of transgene expression levels. We describe the stringently regulated and highly responsive dexamethasone-inducible gene expression system pOp6/LhGR, which comprises the chimeric transcription activator LhGR and the corresponding pOp6 promoter. Upon induction, the LhGR activator binds to the pOp6 promoter and induces expression of the target gene of interest. We provide detailed protocols for inducing transgene expression at different developmental stages and in different plant species and discuss dexamethasone stability and use of its analogs. We also introduce new, versatile, GATEWAY-compatible binary vectors that are now available for the pOp6/LhGR system. © 2019 by John Wiley & Sons, Inc.