Distribution of two splice variants of the glutamate transporter GLT1 in the retinas of humans, monkeys, rabbits, rats, cats, and chickens
Distribution of two splice variants of the glutamate transporter GLT1 in the retinas of humans, monkeys, rabbits, rats, cats, and chickens
复制标题
谷氨酸转运蛋白 GLT1 的两种剪接变体在人、猴、兔、大鼠、猫和鸡视网膜中的分布
DOI：
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
D. Pow
中科院分区：
文献类型：
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作者：
P. Reye;R. Sullivan;E. Fletcher;D. Pow
已经产生了针对谷氨酸转运体GLT1的两个羧基末端剪接变体的抗体，即最初描述的GLT1和GLT1-B版本，并在包括鸡和人类在内的多个物种中进行了标记检测。虽然在每个物种中都观察到了强烈的特异性标记，但注意到了不同的表达模式。此外，每种抗体对适当蛋白质的磷酸化状态都很敏感，因为在大多数情况下，使用碱性磷酸酶化学去除磷酸盐显示了更广泛的标记元件。一般来说，GLT1-B存在于兔、大鼠和猫视网膜的视锥感光细胞和视锥双极细胞中。在鸡和猴的视锥控制的视网膜中，GLT1-B仅与视锥感受器相关，而在猕猴和人的视网膜中，GLT1-B与双极细胞和光感受器的终末相关。在一些物种中，如猫，GLT-B也存在于水平细胞中。相比之下，GLT1的分布各不相同。GLT1在鸡、大鼠、猫和兔子中与无长突细胞相关，在猕猴和猕猴中与双极细胞相关。在大鼠视网膜中，视杆感光终末也含有GLT1，但这只在酶去磷酸化的组织中才明显。我们的结论是，GLT1的两个变体在所有被研究的物种中都存在，但以物种特有的方式存在差异分布。此外，每种细胞类型通常只表达GLT1的一个剪接变体。J.Comp.神经。445：1-12,2002。©2002 Wiley-Liss公司
Antibodies have been generated against two carboxyl‐terminal splice variants of the glutamate transporter GLT1, namely, the originally described version of GLT1 and GLT1‐B, and labelling has been examined in multiple species, including chickens and humans. Although strong specific labelling was observed in each species, divergent patterns of expression were noted. Moreover, each antibody was sensitive to the phosphorylation state of the appropriate protein, because chemical removal of phosphates using alkaline phosphatase revealed a broader range of labelled elements in most cases. In general, GLT1‐B was present in cone photoreceptors and in rod and cone bipolar cells in the retinas of rabbits, rats, and cats. In the cone‐dominated retinas of chickens and in marmosets, GLT1‐B was associated only with cone photoreceptors, whereas, in macaque and human retinas, GLT1‐B was associated with bipolar cells and terminals of photoreceptors. In some species, such as cats, GLT‐B was also present in horizontal cells. By contrast, GLT1 distribution varied. GLT1 was associated with amacrine cells in chickens, rats, cats, and rabbits and with bipolar cells in marmosets and macaques. In the rat retina, rod photoreceptor terminals also contained GLT1, but this was evident only in enzymatically dephosphorylated tissues. We conclude that the two variants of GLT1 are present in all species examined but are differentially distributed in a species‐specific manner. Moreover, each cell type generally expresses only one splice variant of GLT1. J. Comp. Neurol. 445:1–12, 2002. © 2002 Wiley‐Liss, Inc.
影响因子：
2.9
作者：
DANBOLT, NC;PINES, G;KANNER, BI
通讯作者：
KANNER, BI