ABSCISIC-ACID CONTENT OF ALGAE UNDER STRESS
ABSCISIC-ACID CONTENT OF ALGAE UNDER STRESS
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DOI：
10.1111/j.1438-8677.1989.tb00113.x
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发表时间：
1989-11-01
期刊：
影响因子：
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通讯作者：
GIMMLER, H
中科院分区：
文献类型：
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作者：
HIRSCH, R;HARTUNG, W;GIMMLER, H
采用酶联免疫分析法（ELISA）研究了植物激素脱落酸（ABA）在藻门内的分布。64种被测藻类（包括原核生物）均含有ABA，而大肠杆菌、红红螺旋菌和盐杆菌中均未检测到ABA。由此可见，ABA在藻类中是普遍存在的，并不局限于苔藓植物。即使在原核生物中，合成ABA的能力也一定已经发展起来了。本文通过调查研究了ABA在一些选定藻类中的生理作用。2. ABA在细胞与培养基间的分布；2 .内源ABA对胁迫的响应；3 .外源14c -甲戊酸合成14C-ABA；4 . ABA的代谢；外施ABA对藻类各种生理反应的影响，以及去氟唑松对ABA含量的影响。在杜氏藻细胞中，14c -甲基戊酸是14C-ABA合成的前体，ABA被代谢成与高等植物中相同的产物。在高渗透盐冲击下，小孢子虫体内ABA水平升高，在培养基碱化后，嗜酸孢子虫体内ABA水平升高。诺氟唑松使杜氏藻中ABA含量升高。外施ABA对细胞的光合作用、呼吸作用和K+含量没有影响。ABA作用后，嗜酸菌质膜对水的渗透性略有降低。讨论了ABA在藻类中可能的生理功能。
The distribution of the phytohormone, abscisic acid (ABA), within the phylum of Phycophyta was investigated by an enzyme-linked immunoassay (ELISA). Of 64 algal species tested (originating from 9 divisions, 20 classes and 36 orders, including procaryotes) all species contained ABA, whereas no ABA could be detected in the bacteria Escherichia coli, Rhodospirillum rubrum, and Halobacterium halobium. It is concluded that ABA is universally distributed within the algal kingdom and is not restricted to cormophytes. The ability to synthesize ABA must have been developed even within the procaryotes. The physiological role of ABA in some selected algae was studied by investigating 1. the distribution of ABA between the cells and the culture medium, 2. the responses of endogenous ABA to stress, 3. the synthesis of 14C-ABA from externally applied 14C-mevalonic acid, 4. the metabolism of ABA, 5. the effect of externally applied ABA on various physiological reactions of the algae, and the effect of norflurazon on ABA content. 14C-mevalonic acid served as precursor of 14C-ABA synthesis in Dunaliella cells and ABA was metabolised to the same products which have been observed in higher plants. In D. parva the internal ABA level increased upon hyperosmotic salt shocks, and in D. acidophila upon alkalization of the medium. Norflurazon caused an increase of ABA content in Dunaliella. Externally applied ABA did not affect photosynthesis, respiration and K+ content of the cells. The permeability of the plasma membrane of D. acidophila to water was slightly decreased by ABA. The possible physiological function of ABA in algae is discussed.