EVIDENCE FOR ANIONIC CATION-TRANSPORT OF LITHIUM, SODIUM AND POTASSIUM ACROSS HUMAN ERYTHROCYTE-MEMBRANE INDUCED BY DIVALENT ANIONS
EVIDENCE FOR ANIONIC CATION-TRANSPORT OF LITHIUM, SODIUM AND POTASSIUM ACROSS HUMAN ERYTHROCYTE-MEMBRANE INDUCED BY DIVALENT ANIONS
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DOI：
10.1113/jphysiol.1978.sp012454
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发表时间：
1978-01-01
影响因子：
5.5
通讯作者：
DUHM, J
中科院分区：
文献类型：
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作者：
BECKER, BF;DUHM, J
二价阴离子碳酸根、亚硫酸根、草酸根、亚磷酸根和丙二酸根可加速Li+和Na+的被动净转运。此外，邻苯二甲酸盐、马来酸盐、硫酸盐和琥珀酸盐刺激K+的下坡运输。观察到阴离子功效和选择性的显著差异。这些碳酸根型阴离子的作用是可逆的，并可被SITS [4-乙酰氨基-4“-异硫氰酸基-二苯乙烯-2，2”-二磺酸]、双嘧达莫和其他阴离子转移抑制剂完全阻断。双嘧达莫可抑制水杨酸、苯甲酸、硫氰酸盐和2，4-二硝基苯酚引起的阳离子转运加速作用，而SITS对阳离子转运无影响。大量的一价和多价阴离子没有检测到的影响，锂+运输。Li+净吸收诱导草酸盐表现出类似的pH值依赖性的卤化物自我交换报告。碳酸盐型阴离子的运输加速显示出线性，1：1的阴离子和阳离子的浓度依赖性和对称的相对于2侧的膜。二价碳酸盐型阴离子与相应的碱金属阳离子形成单电荷负1：1离子对，离子对通过阴离子交换途径穿过红细胞膜。这种阴离子阳离子运输的概念得到了物理证据的支持，在水介质中形成的一些考虑的离子对。多价阴离子的相对功效和阳离子选择性在很大程度上可以根据控制离子对形成的静电相互作用来解释。螯合性能，结构的灵活性，极化的阴离子和离子对的阴离子交换途径的可及性也需要考虑。一个交换.**图形 **。内部离子对。**图形 **。或Cl-作为细胞pH调节的可能模式进行了讨论。
The passive net transport of Li+ and Na+ across the human red cell membrane was accelerated by the divalent anions carbonate, sulfite, oxalate, phosphite and malonate. Phthalate, maleate, sulfate and succinate additionally stimulated downhill transport of K+. Marked differences in anion efficacy and selectivity were observed. The effects of these carbonate-type anions were reversible and fully blocked by SITS [4-acetamido-4''-isothiocyanato-stilbene-2,2''-disulfonic acid], dipyridamole and other inhibitors of anion transfer. Cation transport acceleration induced by the monovalent anions salicylate, benzoate, thiocyanate and 2,4-dinitrophenol were inhibited by dipyridamole, but not affected by SITS. A great number of mono- and polyvalent anions were without detectable influence on Li+ transport. Li+ net uptake induced by oxalate exhibited a pH dependence similar to that reported for halide self exchange. Transport acceleration by carbonate type anions displayed a linear, 1:1 dependence on the concentrations of the anion and the cation and was symmetric with respect to the 2 sides of the membrane. The divalent carbonate type anions form singly charged, negative 1:1 ion pairs with the respective alkali metal cations, the ion pairs traversing the red cell membrane via the anion exchange pathway. This concept of anionic cation transport is supported by physical evidence obtained in aqueous media for formation of some of the ion pairs considered. The relative efficacies and cation selectivities of polyvalent anions can largely be explained on the basis of electrostatic interactions governing ion pair formation. The chelating properties, structural flexibility, polarizability of the anions and the accessibility of the ion pairs to the anion exchange pathway need also be considered. An exchange of .**GRAPHIC**. ion pairs for internal .**GRAPHIC**. or Cl- is discussed as a possible mode of cellular pH regulation.