NUCLEOTIDE-SEQUENCE AND ORGANIZATION OF THE MOUSE ADENINE PHOSPHORIBOSYLTRANSFERASE GENE - PRESENCE OF A CODING REGION COMMON TO ANIMAL AND BACTERIAL PHOSPHORIBOSYLTRANSFERASES THAT HAS A VARIABLE INTRON EXON ARRANGEMENT
NUCLEOTIDE-SEQUENCE AND ORGANIZATION OF THE MOUSE ADENINE PHOSPHORIBOSYLTRANSFERASE GENE - PRESENCE OF A CODING REGION COMMON TO ANIMAL AND BACTERIAL PHOSPHORIBOSYLTRANSFERASES THAT HAS A VARIABLE INTRON EXON ARRANGEMENT
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DOI：
10.1073/pnas.82.9.2731
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发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
STAMBROOK, PJ
中科院分区：
文献类型：
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作者：
DUSH, MK;SIKELA, JM;STAMBROOK, PJ
测定了小鼠腺嘌呤磷酸核糖基转移酶(Aprt)基因及其c[互补]DNA的核苷酸序列。该酶的氨基酸序列是从cDNA中的一个开放阅读框推导出来的，并预测了一个相对分子质量为19560的蛋白质。该基因的蛋白质编码区为apprxeq。2kb，由5个外显子和4个内含子组成。ATG翻译起始密码子上游的200个核苷酸为66%的G+C，含有3个拷贝的C-C-G-C-C-C。小鼠APRT酶与小鼠、仓鼠和人次黄嘌呤磷酸核糖转移酶(HPRT)以及几种细菌磷酸核糖转移酶具有20个氨基酸的同源序列。该序列可能是人HPRT和大肠杆菌谷氨酰胺磷酸核糖基转移酶的催化结构域。由于这些真核和原核蛋白质在功能上的相似性，它们表现出一个或多个同源区域并不令人意外，特别是在5-磷酸核糖基-1-焦磷酸和嘌呤结合部位，特别是如果它们通过共同的进化谱系联系在一起的话。这个同源序列被小鼠aprt基因中的一个内含子和小鼠hprt基因中的两个内含子打断。两个内含子在hprt序列中的位置与单个内含子在aprt基因相应序列中的位置不同。
The nucleotide sequence of a functional mouse adenine phosphoribosyltransferase (APRT) gene and its c[complementary]DNA was determined. The amino acid sequence of the enzyme is deduced from an open reading frame in the cDNA and predicts a protein with a MW of 19,560. The protein coding region of the gene is .apprxeq. 2 kilobases, and it is composed of 5 exons and 4 introns. While the body of the gene is 53% G + C, the 200 nucleotides upstream from the ATG translation start codon are 66% G + C and contain 3 copies of the sequence C-C-G-C-C-C. The mouse APRT enzyme shares a homologous 20-amino acid sequence with mouse, hamster and human hypoxanthine phosphoribosyltransferases (HPRT) and several bacterial phosphoribosyltransferases. This sequence was previously shown to be a likely catalytic domain in human HPRT and Escherichia coli glutamine phosphoribosyltransferase. Because of the similarities in function of these proteins, both eukaryotic and prokaryotic, it is not unexpected that they should exhibit one or more regions of homology, particularly at the 5-phosphoribosyl-1-pyrophosphate and purine binding sites, especially if they are related via a common evolutionary lineage. This homologous sequence is interrupted by a single interon in the mouse APRT gene and by 2 introns in the mouse HPRT gene. The positions of both introns in the HPRT sequence are different from that of the single intron in the corresponding sequence of the APRT gene.