Evolutionary Conservation of ABA Signaling for Stomatal Closure
Evolutionary Conservation of ABA Signaling for Stomatal Closure
复制标题
气孔关闭 ABA 信号的进化保守
DOI：
10.1104/pp.16.01848
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发表时间：
2017-06-01
期刊：
影响因子：
7.4
通讯作者：
Chen, Zhong-Hua
中科院分区：
文献类型：
--
作者：
Cai, Shengguan;Chen, Guang;Chen, Zhong-Hua
据报道，脱落酸（阿坝）驱动的气孔调节演化后，蕨类植物的分歧，在种子植物的早期进化大约3.6亿年前。这一假说是基于观察到某些蕨类植物的气孔对阿坝无反应，但表现出被动的水力控制。然而，ABA诱导的气孔关闭在一些苔藓和石松中检测到。在这里，我们观察到一些阿坝信号和膜转运蛋白家族多样化的陆地植物的进化历史。水生蕨类植物细叶满江红和萨尔瓦多cucullata有23个家族的蛋白质的代表，正交的拟南芥（拟南芥）和所有其他陆地植物物种的研究。系统发育分析的关键阿坝信号蛋白表明进化保守的气孔响应阿坝。此外，比较转录组学分析已经确定了一套ABA反应基因，差异表达的陆生蕨类植物，Polystichum proliferum。这些基因编码与阿坝生物合成、转运、接收、转录、信号传导、离子和糖转运相关的蛋白，这些蛋白符合拟南芥和大麦构建的阿坝信号传导途径。这些关键的ABA反应基因的保留可能对蕨类植物适应干旱条件产生了深远的影响。此外，气孔测定显示了ABA诱导两种陆生蕨类植物P. proliferum和肾蕨（Nephrolepis exaltata）气孔关闭的初步证据。总之，我们报告，据我们所知，新的分子和生理证据的存在，积极的气孔控制蕨类植物。
Abscisic acid (ABA)-driven stomatal regulation reportedly evolved after the divergence of ferns, during the early evolution of seed plants approximately 360 million years ago. This hypothesis is based on the observation that the stomata of certain fern species are unresponsive to ABA, but exhibit passive hydraulic control. However, ABA-induced stomatal closure was detected in some mosses and lycophytes. Here, we observed that a number of ABA signaling and membrane transporter protein families diversified over the evolutionary history of land plants. The aquatic ferns Azolla filiculoides and Salvinia cucullata have representatives of 23 families of proteins orthologous to those of Arabidopsis (Arabidopsis thaliana) and all other land plant species studied. Phylogenetic analysis of the key ABA signaling proteins indicates an evolutionarily conserved stomatal response to ABA. Moreover, comparative transcriptomic analysis has identified a suite of ABA-responsive genes that differentially expressed in a terrestrial fern species, Polystichum proliferum. These genes encode proteins associated with ABA biosynthesis, transport, reception, transcription, signaling, and ion and sugar transport, which fit the general ABA signaling pathway constructed from Arabidopsis and Hordeum vulgare. The retention of these key ABA-responsive genes could have had a profound effect on the adaptation of ferns to dry conditions. Furthermore, stomatal assays have shown the primary evidence for ABA-induced closure of stomata in two terrestrial fern species P. proliferum and Nephrolepis exaltata. In summary, we report, to our knowledge, new molecular and physiological evidence for the presence of active stomatal control in ferns.