Rehydration Capacities and Rates for Various Porcine Tissues after Dehydration
Rehydration Capacities and Rates for Various Porcine Tissues after Dehydration
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DOI：
10.1371/journal.pone.0072573
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发表时间：
2013-09-04
期刊：
影响因子：
3.7
通讯作者：
Jiang, Jack
中科院分区：
文献类型：
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作者：
Meyer, Jacob P.;McAvoy, Kieran E.;Jiang, Jack
液体对组织生物力学的双相效应在软骨和声带中是众所周知的，但在其他组织类型中并不广泛。过去的研究表明，组织脱水会显著影响生物力学性能，并且再水化可以在某些组织类型中恢复这些性能。然而，这些研究未能考虑暂时暴露于脱水剂或复水剂如何改变组织复水能力，因为过度脱水可能永久性地阻止复水至初始液体体积。将选定的猪组织脱水，直至达到其初始质量的100%至40%。使每个样品在0.9%盐水溶液中再水化5小时，并计算初始和再水化质量值之间的百分比变化。斯皮尔曼相关性检验表明，当组织先前暴露于更高水平的脱水时，尽管进行了再水化，但质量损失更大。此外，Pearson相关性检验表明，当先前暴露于较高水平的脱水时，完全再水化后样品的总液体质量降低。通过将组织脱水至其初始质量的40%，然后在0.9%盐水溶液中再水化60分钟，以15分钟的间隔进行质量测量，来确定再水化率。所有组织的再水化都是非线性的，在初始浸泡后30分钟内，大部分组织的质量显著增加。这项研究表明，组织再水合的能力取决于初始脱水暴露的水平。因此，体外再水化实验需要控制剂量和时间暴露于脱水剂和再水化剂，以避免不完全再水化，并且在水化模型中组合不同组织类型时应谨慎。
The biphasic effects of liquid on tissue biomechanics are well known in cartilage and vocal folds, yet not extensively in other tissue types. Past studies have shown that tissue dehydration significantly impacts biomechanical properties and that rehydration can restore these properties in certain tissue types. However, these studies failed to consider how temporal exposure to dehydrating or rehydrating agents may alter tissue rehydration capacity, as overexposure to dehydration may permanently prevent rehydration to the initial liquid volume. Select porcine tissues were dehydrated until they reached between 100% and 40% of their initial mass. Each sample was allowed to rehydrate for 5 hours in a 0.9% saline solution, and the percent change between the initial and rehydrated mass values was calculated. Spearman correlation tests indicated a greater loss in mass despite rehydration when tissues were previously exposed to greater levels of dehydration. Additionally, Pearson correlation tests indicated the total liquid mass of samples after complete rehydration decreased when previously exposed to higher levels of dehydration. Rehydration rates were found by dehydrating tissues to 40% of their initial mass followed by rehydration in a 0.9% saline solution for 60 minutes, with mass measurements occurring in 15 minute intervals. All tissues rehydrated nonlinearly, most increasing significantly in mass up to 30 minutes after initial soaking. This study suggests the ability for tissues to rehydrate is dependent on the level of initial dehydration exposure. In vitro rehydration experiments therefore require controlled dosage and temporal exposure to dehydrating and rehydrating agents to avoid incomplete rehydration, and caution should be taken when combining different tissue types in models of hydration.