Distribution and electrophoretic properties of alcohol-soluble proteins in normal and high-lysine sorghums
Distribution and electrophoretic properties of alcohol-soluble proteins in normal and high-lysine sorghums
复制标题
正常和高赖氨酸高粱中醇溶性蛋白质的分布和电泳特性
DOI：
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发表时间：
1979
期刊：
影响因子：
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通讯作者：
J. Wall
中科院分区：
文献类型：
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作者：
J. Paulis;J. Wall
比较了正常赖氨酸高粱籽粒和高赖氨酸高粱籽粒的醇溶蛋白的电泳谱和氨基酸组成。用改进的Osborne-Mendel方案对谷粉进行的连续提取证实了这样的发现：与正常高粱相比，高赖氨酸高粱含有更多的盐溶和酒精不溶的还原谷蛋白，而更少的醇溶蛋白质、kafirin和醇溶还原谷蛋白(ASRG)。Kafirin和ASRG在每个品种中的氨基酸组成相似，仅在品种之间略有差异。在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳中，Kafirin和ASRG多肽主要以明显的宽带形式迁移，表观分子量为22,000，但在8M尿素-乳酸铝pH 3.1的凝胶中，Kafirin和ASRG多肽是不均匀的。近等基因正常和高赖氨酸基因型的Kafirin和ASRGs的电泳谱相似，但在无关品种之间不同。这些数据表明Kafirins和ASRG蛋白之间存在密切关系。在遗传研究中，聚丙烯酰胺凝胶电泳法可用于高粱醇溶蛋白的鉴定、比较和区分。
Electrophoretic patterns and amino acid compositions were compared between alcohol-soluble proteins from normal and high-lysine sorghum grain meals. Sequential extraction of grain meals by a modified Osborne-Mendel scheme confirmed findings that the high-lysine sorghums contained more salt-soluble and alcohol-insoluble reduced glutelins and less alcohol-soluble proteins, kafirin and alcohol-soluble reduced glutelin (ASRG) than did the normal sorghums. Kafirin and ASRG had similar amino acid compositions in each variety and differed only slightly between varieties. Kafirin and ASRG polypeptides migrated primarily as a prominent broad band with an apparent MW of 22,000 during sodium dodecyl sulfate-polyacrylamide gel electrophoresis, but they were heterogeneous by polyacrylamide gel electrophoresis in 8 M urea-aluminum lactate pH 3.1. Electrophoretic patterns of kafirins and ASRGs for near-isogenic normal and high-lysine genotypes were similar, but different, between unrelated varieties. These data suggest a close relationship between kafirins and ASRG proteins. Polyacrylamide gel electrophoresis is useful for characterizing, comparing and differentiating sorghum alcohol-soluble proteins in genetic investigations.