Inhibition of off-target cleavage by RNase H using an artificial cationic oligosaccharide
Inhibition of off-target cleavage by RNase H using an artificial cationic oligosaccharide
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使用人工阳离子寡糖抑制 RNase H 的脱靶裂解
DOI：
10.1039/d1ob00983d
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发表时间：
2021
影响因子：
3.2
通讯作者：
Takeshi Wada
中科院分区：
文献类型：
--
作者：
Rintaro Iwata Hara;Takeshi Wada
序列依赖性脱靶效应是基于反义寡核苷酸的药物的一个严重问题。其中一些副作用是由核糖核酸酶 H (RNase H) 介导的碱基序列与靶 RNA 相似的非靶 RNA 的切割引起的。我们发现人工阳离子寡糖 ODAGal4 改善了 RNase H 切割的单碱基辨别能力。
Sequence-dependent off-target effects are a serious problem of antisense oligonucleotide-based drugs. Some of these side effects are induced by ribonuclease H (RNase H)-mediated cleavage of non-target RNAs with base sequences similar to that of the target RNA. We found that an artificial cationic oligosaccharide, ODAGal4, improved single-base discrimination for RNase H cleavage.