The soluble urokinase plasminogen activator receptor and its fragments in venous ulcers
The soluble urokinase plasminogen activator receptor and its fragments in venous ulcers
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DOI：
10.1016/j.jvsv.2014.08.002
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发表时间：
2015-04-01
影响因子：
3.2
通讯作者：
Smith, Alberto
中科院分区：
文献类型：
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作者：
Ahmad, Anwar;Saha, Prakash;Smith, Alberto
目的：通过尿激酶型纤溶酶原激活物(UPA)与其受体uPAR结合，激活细胞表面的蛋白分解机制，是创面愈合的重要过程。可溶性uPAR(suPAR.)及其片段I、H和III)具有非蛋白水解性功能，包括趋化、黏附和增殖，这些功能也在伤口愈合中发挥作用。本研究的目的是确定静脉性溃疡中是否存在suPAR及其裂解片段，以及它们的水平是否与愈合有关。方法：收集30例静脉性小腿溃疡患者的溃疡渗出液。愈合定义为压迫治疗后6个月内完全再上皮化。时间分辨荧光免疫分析法可定量检测溃疡渗出液中的suPAR及其片段。结果：溃疡渗出液中完整的suPAR水平是正常水平的3倍(P=.005)，Supari水平是正常水平的2倍(P=0.03)，PARH-11水平大约是PARH-11水平的3倍(P=0.03)。
Objective: Activation of proteolytic mechanisms at the cell surface through the activity of urokinase-type plasminogen activator (uPA) bound to its receptor, uPAR, is an important process in wound healing. The soluble forms of uPAR (suPAR. and its fragments I, H, and III) have nonproteolytic functions that include chemotaxis, adhesion, and proliferation, which also have a role in wound healing. The aim of this study was to determine whether suPAR and its cleaved fragments are present in venous ulcers and whether their levels are associated with healing.Methods: Ulcer exudates were collected from patients with venous leg ulcers (n = 30). Healing was defined as complete re-epithelialization within 6 months of compression therapy. Time -resolved fluorescence immunoassays were validated for quantification of suPAR and its fragments in ulcer exudates. The effect of exudates on keratinocyte migration was analyzed by an in vitro scratch assay.Results: Ulcer exudates from patients who healed (n = 9) had approximately threefold higher levels of intact suPAR (P =.005), twofold higher levels of suPARI (P =.03), and approximately threefold higher levels suPARH-Ill (P