A population balance model describing the cell cycle dynamics of myeloma cell cultivation
A population balance model describing the cell cycle dynamics of myeloma cell cultivation
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DOI：
10.1021/bp070152z
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发表时间：
2007-09-01
影响因子：
2.9
通讯作者：
Yuan, Jing-Qi
中科院分区：
文献类型：
--
作者：
Liu, Yuan-Hua;Bi, Jing-Xiu;Yuan, Jing-Qi
提出了多阶段群体平衡模型来描述骨髓瘤细胞培养的细胞周期动态。在该模型中，细胞周期分为三个阶段，即G1期、S期和G2M期。 DNA 含量和细胞体积都用于将每个细胞与群体中的其他细胞区分开来。假设从 G1 到 S 的转变和 G2M 分裂的概率取决于细胞体积，从 S 到 G2M 的转变概率由 DNA 含量决定。该模型可用于模拟 DNA 含量和细胞体积分布、相分数、底物和副产物浓度以及细胞密度的动态。骨髓瘤细胞培养的测量，尤其是不同阶段的 DNA 分布和细胞分数的 FACS 数据，用于模型验证。
A multi-staged population balance model is proposed to describe the cell cycle dynamics of myeloma cell cultivation. In this model, the cell cycle is divided into three stages, i.e., G1, S, and G2M phases. Both DNA content and cell volume are used to differentiate each cell from other cells of the population. The probabilities of transition from G1 to S and division of G2M are assumed to be dependent on cell volume, and transition probability from S to G2M is determined by DNA content. The model can be used to simulate the dynamics of DNA content and cell volume distributions, phase fractions, and substrate and byproduct concentrations, as well as cell densities. Measurements from myeloma cell cultivations, especially the FACS data with respect to DNA distribution and cell fractions in different stages, are employed for model validation.