INSULIN REMOVAL BY ISOLATED PERFUSED RAT-LIVER
INSULIN REMOVAL BY ISOLATED PERFUSED RAT-LIVER
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DOI：
10.1152/ajplegacy.1976.230.1.171
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发表时间：
1976-01-01
影响因子：
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通讯作者：
LOWENSTEIN, JM
中科院分区：
文献类型：
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作者：
MISBIN, RI;MERIMEE, TJ;LOWENSTEIN, JM
在灌流的大鼠肝脏中研究了未标记胰岛素的去除。胰岛素的清除在吸收后大鼠的门静脉浓度范围内遵循一级动力学，但在更宽的浓度范围内的实验中偏离了一级动力学。在吸收后状态下，门静脉内正常浓度(0.40-1.1 nM或约60-100微米/毫升)的清除量是胰腺刺激后预期浓度(4.5-7.0毫微米)的两倍以上。然而，即使在更高的水平上，也没有观察到肝脏清除胰岛素的能力饱和。当流速降低时，胰岛素清除率降低。长时间的饥饿并没有显著改变它。我们的结果表明，当胰岛素浓度较高时，与较低浓度时相比，更大比例的人逃脱了肝脏的降解。肝脏对胰岛素的清除在一定程度上依赖于门脉血流量。通过灌流的肝脏去除胰岛素的动力学不能用其他人所描述的胰岛素降解酶的性质来解释。
Removal of unlabeled insulin was studied in the perfused rat liver. Insulin removal followed first-order kinetics over the range of concentrations found in the portal vein of postabsorptive rats, but deviated from first-order kinetics in experiments with a wider concentration range. Clearance was more than twice as great at concentrations normally found in the portal vein in the postabsorptive state (0.40-1.1nM or about 60-100 muU/ml) than at concentrations expected after pancreatic stimulation (4.5-7.0 nM). Saturation of the liver's capacity to remove insulin, however, was not observed even at higher levels. Insulin clearance diminished when the flow rate was reduced. It was not significantly altered by prolonged starvation. Our results suggest that when the insulin concentration is high a greater percentage escapes hepatic degradation then when it is low. Hepatic insulin clearance is in part dependent on the portal flow rate. The kinetics of insulin removal by the perfused liver cannot be accounted for by the properties of insulin-degrading enzymes described by others.