Inhibitory properties of the antibody response to Plasmodium vivax Duffy binding protein in an area with unstable malaria transmission
Inhibitory properties of the antibody response to Plasmodium vivax Duffy binding protein in an area with unstable malaria transmission
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DOI：
10.1111/j.1365-3083.2007.02059.x
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发表时间：
2008-03-01
影响因子：
3.7
通讯作者：
Carvalho, L. H.
中科院分区：
文献类型：
--
作者：
Ceravolo, I. P.;Souza-Silva, F. A.;Carvalho, L. H.
间日疟原虫Duffy结合蛋白（DBP）在红细胞侵入过程中的功能对于寄生虫在人类感染中的成功生长和发病至关重要。尽管DBP是深入的疟疾疫苗研究的主题，但对人群中抗DBP抗体的功能特性的研究有限[Infect Immun 68（2000）3164]。在本研究中，我们研究了来自巴西亚马逊地区不同人群的血清的能力-一个显着不稳定的疟疾传播-抑制DBP配体结构域（区域II，DBPII）的红细胞结合功能。我们发现，在亚马逊地区长期接触疟疾会引发DBP特异性抗体，这些抗体会抑制不同DBPII变体与红细胞的结合。尽管在研究参与者中观察到的抑制性抗体反应存在很大差异，但我们观察到红细胞结合抑制活性与酶联免疫吸附测定抗DBP抗体之间存在正相关性。重要的是，在无症状间日疟原虫感染的个体中，抗DBP抗体水平升高的趋势不显著。
The function of the Plasmodium vivax Duffy binding protein (DBP) during the erythrocyte invasion process is critical for successful parasite growth and pathogenesis in human infections. Although DBP is the subject of intensive malaria vaccine research, investigations on the functional proprieties of anti-DBP antibodies in the human population have been limited [Infect Immun68 (2000) 3164]. In the present study, we examined the ability of sera from different populations of the Brazilian Amazon - an area of markedly unstable malaria transmission - to inhibit the erythrocyte-binding function of the DBP ligand domain (region II, DBPII). We found that long-term exposure to malaria in the Amazon area elicits DBP-specific antibodies that inhibit the binding of different DBPII variants to erythrocytes. Despite the great variability of inhibitory antibody responses observed among study participants, we observed a positive correlation between erythrocyte binding-inhibitory activity and enzyme-linked immunosorbent assay anti-DBP antibodies. Of importance, there was a non-significant tendency towards increased levels of anti-DBP antibodies among individuals with asymptomatic P. vivax infections.