Super-resolution photon-efficient imaging by nanometric double-helix point spread function localization of emitters (SPINDLE)
Super-resolution photon-efficient imaging by nanometric double-helix point spread function localization of emitters (SPINDLE)
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DOI：
10.1364/oe.20.026681
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发表时间：
2012-11-19
期刊：
影响因子：
3.8
通讯作者：
Piestun, Rafael
中科院分区：
文献类型：
--
作者：
Grover, Ginni;DeLuca, Keith;Piestun, Rafael
光激活或光切换探针的超分辨率成像在生物学应用中是一种很有前途的工具，可以用可见光揭示以前未解决的细胞内细节。这一领域得益于分子探针、光学系统和数据的计算后处理领域的发展。使用双螺旋点扩展函数（DH-PSF）的光学和重建过程的联合设计提供了长景深的高分辨率三维（3D）成像。我们首次展示了一种集成Fisher信息高效DH-PSF设计，表面浮雕光学相位掩模和最佳3D定位估计器的方法。利用光可切换染料的三维超分辨率成像揭示了哺乳动物细胞中的三维微管网络，其定位精度接近信息理论极限，深度为1.2 μ m。(C) 2012光学学会美国
Super-resolution imaging with photo-activatable or photo-switchable probes is a promising tool in biological applications to reveal previously unresolved intra-cellular details with visible light. This field benefits from developments in the areas of molecular probes, optical systems, and computational post-processing of the data. The joint design of optics and reconstruction processes using double-helix point spread functions (DH-PSF) provides high resolution three-dimensional (3D) imaging over a long depth-of-field. We demonstrate for the first time a method integrating a Fisher information efficient DH-PSF design, a surface relief optical phase mask, and an optimal 3D localization estimator. 3D super-resolution imaging using photo-switchable dyes reveals the 3D microtubule network in mammalian cells with localization precision approaching the information theoretical limit over a depth of 1.2 mu m. (C) 2012 Optical Society of America