Magnesium ions but not ATP inhibit cyclic ADP-ribose-induced calcium release.
Magnesium ions but not ATP inhibit cyclic ADP-ribose-induced calcium release.
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镁离子而非 ATP 抑制环状 ADP-核糖诱导的钙释放。
DOI：
10.1006/bbrc.1995.1111
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Lee,HC
中科院分区：
文献类型：
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作者：
Graeff,RM;Podein,RJ;Aarhus,R;Lee,HC
环 ADP 核糖 (cADPR) 依赖性 Ca+2 释放机制的药理学与兰尼碱受体 (RyR) 非常相似。在这里，我们表明，MgCl2（一种已知的 RyR 抑制剂）可阻断海胆卵匀浆中 cADPR 诱导的 Ca+2 释放，半数浓度约为 2.5 mM。该效果是特异性的，因为高达 10 mM Mg+2 对肌醇三磷酸诱导的 Ca+2 释放没有影响。 K2ATP（另一种已知的 RyR 调节剂）在高达 10 mM 时不会影响 cADPR 的半最大浓度，该浓度仍保持在约 96 nM。这些结果表明 cADPR 是一种特异性 Ca+2 释放激活剂，而不仅仅是作用于 ATP 位点的腺嘌呤核苷酸。 Mg+2 的抑制作用进一步证明了 RyR 和 cADPR 依赖性 Ca+2 释放系统之间的相似性。
The pharmacology of the cyclic ADP-ribose (cADPR)-dependent Ca+2release mechanism is very similar to that of the ryanodine receptor (RyR). Here we showed that MgCl2, a known inhibitor of RyR, blocked cADPR-induced Ca+2release in sea urchin egg homogenates with a half maximal concentration of about 2.5 mM. The effect was specific since up to 10 mM Mg+2had no effect on the Ca+2release induced by inositol trisphosphate. K2ATP, another known modulator of RyR, at up to 10 mM did not affect the half-maximal concentration of cADPR, which remained at about 96 nM. These results indicate cADPR is a specific Ca+2release activator and not merely an adenine nucleotide acting on the ATP-site. The inhibitory effects of Mg+2further demonstrate the similarity between RyR and the cADPR-dependent Ca+2release system.