Hydrogen peroxide production by Mycoplasma bovis and Mycoplasma agalactiae and effect of in vitro passage on a Mycoplasma bovis strain producing high levels of H2O2
Hydrogen peroxide production by Mycoplasma bovis and Mycoplasma agalactiae and effect of in vitro passage on a Mycoplasma bovis strain producing high levels of H2O2
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DOI：
10.1023/b:verc.0000047506.04096.06
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发表时间：
2005-04-01
影响因子：
2.2
通讯作者：
Nicholas, RAJ
中科院分区：
文献类型：
--
作者：
Khan, LA;Miles, RJ;Nicholas, RAJ
测定了牛支原体和支原体的典型株和田间株在裂解细胞氧化NADH和L-α-甘油磷酸（GP）过程中过氧化氢（H2 O2）的产生和氧摄取。无乳。所有菌株的NADH氧化表现出可变的11202生产范围从0到1.21 mol/mol O-2采取。所有菌株都不能氧化GP，表现出GP氧化酶活性的缺乏。一些菌株被鉴定为产生相对高水平的H2 O2（> 1.0 mol/mol O-2吸收）。M.牛菌株119 B 96显示出减少的H2 O2产生：在第50、100和200次传代后分别吸收0.52、0.16和0.07 mol/mol O-2。SDS-PAGE分析显示，在50次传代后，32 kDa的蛋白条带丢失。这些初步研究表明，不仅H2 O2的生产由潜在的致病性支原体属。这些改变在田间变化，但也可以通过在培养物中传代诱导类似的改变。在后一种情况下，至少在一个M.牛菌株中，SDS-PAGE显示这种改变与特异性蛋白质产生的损失有关。对这些现象的进一步研究是生产更有效的支原体疫苗的必要背景。
Hydrogen peroxide (H2O2) production and oxygen uptake during the oxidation of NADH and L-alpha-glycerophosphate (GP) by lysed cells was determined for the type and field strains of Mycoplasma bovis and M. agalactiae. NADH oxidation by all the strains showed variable production of 11202 ranging from 0 to 1.21 mol/mol O-2 taken up. All strains were unable to oxidize GP, showing absence of GP oxidase activity. Some strains were identified that produced relatively high levels of H2O2 (> 1.0 mol/mol O-2 taken up). In vitro passage of M. bovis strain 119B96 showed reduced H2O2 production: 0.52, 0.16, and 0.07 mol/mol O-2 taken up after the 50th, 100th and 200th passages, respectively. SDS-PAGE analysis showed the loss of a protein band of 32 kDa after 50 passages. These preliminary studies show that not only does H2O2 production by potentially pathogenic Mycoplasma spp. vary in the field but also that similar alterations can be induced by passage in culture. In the latter case, at least in one M. bovis strain, this alteration has been shown by SDS-PAGE to be associated with a loss of specific protein production. Further study of these phenomena is essential background for the production of more efficient vaccines for mycoplasmas.