Amino acid substitution (histidine to tyrosine) in a glucose-6-phosphate dehydrogenase variant (G6PD Hektoen) associated with over-production.
Amino acid substitution (histidine to tyrosine) in a glucose-6-phosphate dehydrogenase variant (G6PD Hektoen) associated with over-production.
复制标题
葡萄糖-6-磷酸脱氢酶变体 (G6PD Hektoen) 中的氨基酸取代（组氨酸变为酪氨酸）与过量生产相关。
DOI：
10.1016/0022-2836(70)90414-6
复制
发表时间：
1970
影响因子：
5.6
通讯作者：
A. Yoshida
中科院分区：
文献类型：
--
作者：
A. Yoshida
正常人葡萄糖-6-磷酸脱氢酶和Hektoen变异体之间的结构差异与四倍增加的酶浓度进行了阐明，其胰蛋白酶和胰凝乳蛋白酶肽的肽图谱。发现了一个单一的氨基酸替换，从正常酶中的组氨酸到变异酶中的酪氨酸，这些发现表明，结构基因中导致氨基酸替换的单步碱基变化也可能增加变异蛋白的产量。
The structural difference between the normal human glucose-6-phosphate dehydrogenase and the Hektoen variant associated with fourfold increased enzyme concentration was elucidated by peptide mapping of their tryptic and chymotryptic peptides. A single amino acid substitution, from histidine in the normal enzyme to tyrosine in the variant enzyme, was found. These findings indicate that a single-step base change in a structural gene resulting in an amino acid substitution may also increase production of the variant protein.