Compatibility in the Biomphalaria glabrata/Echinostoma caproni model:: Potential involvement of adhesion genes
Compatibility in the Biomphalaria glabrata/Echinostoma caproni model:: Potential involvement of adhesion genes
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DOI：
10.1016/j.ijpara.2005.09.009
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发表时间：
2006-02-01
影响因子：
4
通讯作者：
Mitta, G
中科院分区：
文献类型：
--
作者：
Bouchut, A;Roger, E;Mitta, G
由于敏感性或电阻的双脐螺光滑的吸虫棘口吸虫caproni相关的差异血细胞粘附特性，我们比较了敏感和耐药蜗牛的血细胞之间的粘附过程中所涉及的基因的表达。定量逆转录酶-PCR分析显示，四个基因的成绩单之间的差异代表抗性和易感蜗牛的血细胞。这些基因编码两种皮连蛋白样蛋白，一种基质蛋白样蛋白和一种钙粘蛋白样蛋白。寄生虫暴露后进行的表达分析表明，皮连蛋白可能参与这些菌株之间的兼容性差异。我们还研究了可能参与细胞外基质结构或凝血的不同基因的整个蜗牛的表达水平。我们的研究结果支持这一假设，即易感蜗牛具有血淋巴凝固样系统，是更有力的比耐药蜗牛。该系统可以防止血细胞向寄生虫幼虫迁移，从而促进寄生虫在易感蜗牛中的定居。(C)2005年澳大利亚寄生虫学会由爱思唯尔有限公司出版。保留所有权利。
Because susceptibility or resistance of Biomphalaria glabrata to the trematode Echinostoma caproni correlates with differential hemocytic adhesive properties, we compared the expression of genes involved in adhesion processes between hemocytes from Susceptible and resistant snails. Quantitative reverse transcriptase-PCR analysis revealed four genes whose transcripts were differentially represented between hemocytes from resistant and susceptible snails. These genes encode two dermatopontin-like, one matrilin-like and one cadherin-like proteins. Expression analyses performed following parasite exposure suggested that dermatopontins may be involved in the compatibility differences between these strains. We also investigated expression levels on whole snails of different genes potentially involved in extracellular matrix structure or coagulation. Our results support the hypothesis that susceptible snails possess a hemolymph coagulation-like system that is more potent than that of resistant snails. This system may prevent hemocyte migration towards the parasite larvae and therefore facilitate parasite settlement in susceptible snails. (C) 2005 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.