Concentration measurement by proton NMR using the ERETIC method
Concentration measurement by proton NMR using the ERETIC method
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DOI：
10.1021/ac981422i
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发表时间：
1999-07-01
影响因子：
7.4
通讯作者：
Trierweiler, M
中科院分区：
文献类型：
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作者：
Akoka, S;Barantin, L;Trierweiler, M
ERETIC方法（电子参考体内浓度）提供了一个参考信号，由电子设备合成，可用于测定绝对浓度。这里给出的结果证明了该方法在H-1高分辨率NMR的情况下的准确度和精密度。五个试管中填充了浓度范围为5.25至54.11 mM的盐酸三甲胺（TMA）和乳酸钠的D2 O溶液。将ERETIC方法获得的结果与使用TMA作为内部参比获得的结果进行了比较。两种方法的标准差相同，始终低于平均值的1%。ERETIC方法的准确度（真实值和测量值之间的差异）略优于内部参考。当实验进行超过56小时时，未观察到显著变化。在三个月内每月重复测量一次。由于获得的值显示标准偏差仅为3%，因此我们可以得出结论，ERETIC方法具有良好的稳定性，仅需每月校准。此外，必须注意的是，没有任何东西被添加到样本中，并且参考信号频率可以被自由选择以落入频谱的透明区域内。
The ERETIC method (Electronic REference To access In vivo Concentrations) provides a reference signal, synthesized by an electronic device, which can be used for the determination of absolute concentrations. The results presented here demonstrate the accuracy and precision of the method in the case of H-1 high resolution NMR Five tubes were filled with D2O solutions of trimethylamine hydrochloride (TMA) 3.84 mM and sodium lactate at concentrations ranging from 5.25 to 54.11 mM. Results obtained with the ERETIC method were compared to those obtained by using TMA as an internal reference. The standard deviations were the same for the two methods and always lower than 1% of the mean. The accuracy (difference between true value and measured value) was slightly better for the ERETIC method than for the internal reference. No significant variation was observed when the experiments were performed over 56 h. Measurements were repeated once a month during three months. As the values obtained showed a standard deviation of only 3%, we can conclude that the ERETIC method has a good stability and only requires monthly calibration. Furthermore, it must be noted that nothing is added to the sample and that the reference signal frequency can be freely chosen to fall within a transparent region of the spectrum.