Controlling the direction of site-selectivity and regioselectivity in RNA ligation by Zn2+-dependent deoxyribozymes that use 2′,3′-cyclic phosphate RNA substrates
Controlling the direction of site-selectivity and regioselectivity in RNA ligation by Zn2+-dependent deoxyribozymes that use 2′,3′-cyclic phosphate RNA substrates
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DOI：
10.1039/b813566e
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发表时间：
2008-01-01
影响因子：
3.2
通讯作者：
Silverman, Scott K.
中科院分区：
文献类型：
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作者：
Kost, Diana M.;Gerdt, Joseph P.;Silverman, Scott K.
我们以前的工作是通过体外选择来鉴定许多依赖于锌离子的脱氧核酶，这些核酶连接两个RNA底物，在2‘，3’-环磷酸发生反应。每个脱氧核酶产生几种不同的RNA连接中的一种，包括天然的3‘-5’和非天然的2‘-5’磷酸二酯键以及许多非天然的连接。在这份报告中，我们描述了揭示选择策略的设计方面的实验，该选择策略有利于天然3‘-5’RNA连接的位置选择性和区域选择性合成。结果还表明，如果要使脱氧核酶具有实用的通用性，就必须为RNA底物序列的通用性制定一个明确的选择压力。
Our previous efforts have used in vitro selection to identify numerous Zn2+-dependent deoxyribozymes that ligate two RNA substrates with reaction at a 2',3'-cyclic phosphate. Each deoxyribozyme creates one of several different RNA linkages, including native 3'-5' and non-native 2'-5' phosphodiester bonds as well as many unnatural linkages. In this report, we describe experiments to reveal design aspects of the selection strategy that favor site-selective and regioselective synthesis of native 3'-5' RNA linkages. The results also reveal that an explicit selection pressure for RNA substrate sequence generality must be developed if the deoxyribozymes are to have practical generality.