Identification of powdery mildew resistance QTL in Fragaria x ananassa

Identification of powdery mildew resistance QTL in Fragaria x ananassa
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草莓抗白粉病QTL鉴定

DOI:
10.1101/196485
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发表时间:
2018
期刊:
--
影响因子:
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通讯作者:
Cockerton H
Cockerton H
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作者:
Cockerton H

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专性生物营养真菌Podosphaera aphanisis是栽培草莓白粉病的病原菌。在一系列田间试验中,对来自两个双亲作图群体‘Emily’x‘Fenella’和‘Redgauntlet’x‘Hapil’的白粉病严重程度进行了表型鉴定。在这里,我们报告了与白粉病抗性相关的多个QTL,这些QTL是在不同年份和地点进行的10个表型事件中确定的。7个QTL在多个表型事件中表现出稳定的抗性水平,而许多其他QTL在单个表型事件中被表达,因此必须被认为是瞬时的。其中一个已鉴定的QTL与更广泛的种质中的一个相关抗性基因紧密连锁。此外,初步的关联分析发现了一个新的保守基因座,可供进一步研究。我们的数据表明,对白粉病的抗性是高度复杂的,草莓种质中存在多个数量抗白粉病的加性来源。在分子标记辅助育种或基因组选择中应用已报道的标记将有助于改良草莓抗白粉病品种,特别是在所研究的亲本、后代和近缘系谱材料被纳入育种种质的情况下。QTL在更广泛的种质间有一定的可转移性。
The obligate biotrophic fungusPodosphaera aphanisis the causative agent of powdery mildew on cultivated strawberry (Fragaria x ananassa). Genotypes from two bi-parental mapping populations ‘Emily’ x ‘Fenella’ and ‘Redgauntlet’ x ‘Hapil’ were phenotyped for powdery mildew disease severity in a series of field trials. Here we report multiple QTL associated with resistance to powdery mildew, identified in ten phenotyping events conducted across different years and locations. Seven QTL show a level of stable resistance across multiple phenotyping events however many other QTL were represented in a single phenotyping event and therefore must be considered transient. One of the identified QTL was closely linked to an associated resistance gene across the wider germplasm. Furthermore, a preliminary association analysis identified a novel conserved locus for further investigation. Our data suggests that resistance is highly complex and that multiple additive sources of quantitative resistance to powdery mildew exist across strawberry germplasm. Implementation of the reported markers in marker-assisted breeding or genomic selection would lead to improved powdery mildew resistant strawberry cultivars, particularly where the studied parents, progeny and close pedigree material are included in breeding germplasm.Key MessagePowdery mildew resistance in two strawberry mapping populations is controlled by both stable and transient novel QTL of moderate effect. Some transferability of QTL across wider germplasm was observed.
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