Systematic spatial analysis of gene expression during wheat caryopsis development
Systematic spatial analysis of gene expression during wheat caryopsis development
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DOI：
10.1105/tpc.105.034058
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发表时间：
2005-08-01
期刊：
影响因子：
11.6
通讯作者：
Doonan, JH
中科院分区：
文献类型：
--
作者：
Drea, S;Leader, DJ;Doonan, JH
谷物颖果是一个复杂的组织，其中母体组织和胚乳组织遵循不同但协调的发育程序。由于小麦（Triticum aestivum）的基因组是六倍体，通过遗传学方法鉴定与关键发育过程有关的基因一直是困难的。为了绕过这一限制，我们调查了888个基因表达的颖果发育过程中使用一种新的高通量mRNA原位杂交方法。这项调查揭示了新的独特的空间表达模式，反映了发育中的颖果的个体发育或表示专门的细胞功能。我们已经确定了已知的和新的基因，其表达是细胞周期依赖性的。我们已经确定折痕区域在建立发育模式中是重要的，因为从增殖到分化的过渡从这个区域扩展到胚乳的其余部分。这组基因与水稻（Oryza sativa）基因组的比较显示，大约三分之二具有水稻对应物，但也表明在涉及籽粒灌浆的蛋白质方面存在相当大的差异。我们发现小麦基因与350个拟南芥基因有显著的同源性。其中至少有25个已知是拟南芥种子发育所必需的，但许多其他的仍有待鉴定。
The cereal caryopsis is a complex tissue in which maternal and endosperm tissues follow distinct but coordinated developmental programs. Because of the hexaploid genome in wheat ( Triticum aestivum), the identification of genes involved in key developmental processes by genetic approaches has been difficult. To bypass this limitation, we surveyed 888 genes that are expressed during caryopsis development using a novel high- throughput mRNA in situ hybridization method. This survey revealed novel distinct spatial expression patterns that either reflected the ontogeny of the developing caryopsis or indicated specialized cellular functions. We have identified both known and novel genes whose expression is cell cycle - dependent. We have identified the crease region as important in setting up the developmental patterning, because the transition from proliferation to differentiation spreads from this region to the rest of the endosperm. A comparison of this set of genes with the rice ( Oryza sativa) genome shows that approximately two- thirds have rice counterparts but also suggests considerable divergence with regard to proteins involved in grain filling. We found that the wheat genes had significant homology with 350 Arabidopsis thaliana genes. At least 25 of these are already known to be essential for seed development in Arabidopsis, but many others remain to be characterized.