ANALYSIS OF ESCHERICHIA-COLI PROMOTER SEQUENCES
ANALYSIS OF ESCHERICHIA-COLI PROMOTER SEQUENCES
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DOI：
10.1093/nar/15.5.2343
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发表时间：
1987-03-11
影响因子：
14.9
通讯作者：
REYNOLDS, RP
中科院分区：
文献类型：
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作者：
HARLEY, CB;REYNOLDS, RP
我们收集和分析了263个启动子，这些启动子都有已知的转录起点。通过程序对启动子元件(-35个六角体、-10个六角体以及这些区域之间的间距)进行比对，该程序选择与起始点一致并且在统计上与启动子参考列表最同源的排列。最初的参考文献列表是Hawley和McClure(Nucl.酸解11,2237-2255,1983)。在连续的分析没有改变清单的结构之前，完整清单的对比被用作参考。在最终的汇编中，-35(TTGACA)和-10(TATAAT)六聚体中的所有碱基都是高度保守的，92%的启动子的区域间距为17±1bp，75%的唯一定义的起始点起始于-10区域下游的7±1碱基。区域间间隔为16、17或18个碱基的启动子的一致序列没有不同。这种汇编和分析应该用于启动子结构和功能的研究以及识别潜在启动子序列的程序
We have compiled and analyzed 263 promoters vith known transcriptional start points for E. coll genes. Promoter elements (-35 hexamer, -10 hexamer, and spacing between these regions) were aligned by a program which selects the arrangement consistent with the start point and statistically most homologous to a reference list of promoters. The initial reference list was that of Hawley and McClure (Nucl. Acids Res. 11, 2237–2255, 1983). Alignment of the complete list was used for reference until successive analyses did not alter the structure of the list. In the final compilation, all bases in the -35 (TTGACA) and -10 (TATAAT) hexamers were highly conserved, 92% of promoters had inter-region spacing of 17±1 bp, and 75% of the uniquely defined start points initiated 7±1 bases downstream of the -10 region. The consensus sequence of promoters with inter-region spacing of 16, 17, or 18 bp did not differ. This compilation and analysis should be usaful for studies of promoter structure and function and for programs which identify potential promoter sequences