Surface plasmon resonance imaging of excitable cells.
Surface plasmon resonance imaging of excitable cells.
复制标题
可兴奋细胞的表面等离子共振成像。
DOI：
10.1088/1361-6463/aaf849
复制
发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Howe CL
中科院分区：
文献类型：
--
作者：
Howe CL
表面等离子体激元（SPs）是发生在金属/电介质界面的表面电荷密度振荡，并且对邻近表面的折射率变化高度敏感。这种灵敏度已成功地用于化学和生物测定。在这些系统中，基于表面等离子体共振（SPR）的传感器检测一点处的折射率的时间变化。SPR也已用于成像系统中，其中样品中折射率的空间变化提供对比机制。使用高数值孔径（NA）物镜的SPR成像系统已被设计成以高放大率和近衍射极限的空间分辨率对粘附的活细胞进行成像。解决细胞生理学和药理学中的研究问题通常需要开发一种可以获得互补信息的多模态显微镜。
Surface plasmons (SPs) are surface charge density oscillations occuring at a metal/dieletric interface and are highly sensitive to refractive index variations adjacent to the surface. This sensitivity has been exploited successfully for chemical and biological assays. In these systems, a surface plasmon resonance (SPR)-based sensor detects temporal variations in the refractive index at a point. SPR has also been used in imaging systems where the spatial variations of refractive index in the sample provide the contrast mechanism. SPR imaging systems using high numerical aperture (NA) objective lenses have been designed to image adherent live cells with high magnification and near-diffraction limited spatial resolution. Addressing research questions in cell physiology and pharmacology often requires the development of a multimodal microscope where complementary information can be obtained.