Extraction of Puerarin using Ionic Liquid Based Aqueous Two-Phase Systems
Extraction of Puerarin using Ionic Liquid Based Aqueous Two-Phase Systems
复制标题
使用离子液体水两相体系提取葛根素
DOI：
10.1080/01496395.2012.659787
复制
发表时间：
2012-01-01
影响因子：
2.8
通讯作者：
Ouyang, Xiao-Kun
中科院分区：
文献类型：
--
作者：
Fan, Jie-Ping;Cao, Jing;Ouyang, Xiao-Kun
对多种基于离子液体的双水相体系（IL - ATPS）提取葛根素进行了评估。结果表明，离子液体的性质、盐的盐析能力以及富盐溶液的酸碱度对提取效率有重要影响。对各种因素，即离子液体、盐、葛根素和短链醇的用量进行了系统优化。在最佳实验条件下（磷酸氢二钾用量为0.30 - 0.42 g/mL，[Bmim]Br用量为0.30 - 0.36 g/mL以及1.0 mL葛根素储备液），通过单步提取，葛根素的提取效率超过99%，这表明所评估的IL - ATPS是一种有前景的提取介质。最后，IL - ATPS成功用于从野葛提取物中提取葛根素。
Various ionic liquid-based aqueous two-phase systems (IL-ATPS) were evaluated to extract puerarin. The results indicated that the nature of ILs, the salting-out ability of salt, and the acidity and basicity of the salt-rich solution had important influence on the extraction efficiency. Various factors were optimized systematically, that is, the amount of IL, salt, puerarin, and short-chain alcohol. Under the optimal experimental conditions (the amount of K2HPO4 0.30-0.42 g/mL, [Bmim]Br 0.30-0.36 g/mL and 1.0 mL puerarin stock solution), the extraction efficiency of puerarin was over 99% by a single-step extraction, which indicated that the evaluated IL-ATPS was a prospective extraction medium. Finally, the IL-ATPS was successfully used to extract puerarin from Radix Puerariae Lobatae extracts.