Functional conservation of cis-regulatory elements of heat-shock genes over long evolutionary distances.
Functional conservation of cis-regulatory elements of heat-shock genes over long evolutionary distances.
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DOI：
10.1371/journal.pone.0022677
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Ruvinsky I
中科院分区：
文献类型：
--
作者：
He Z;Eichel K;Ruvinsky I
基因调控的转录控制是一个复杂的过程，需要许多分子组分的精确协调。研究它的进化可以作为理解复杂分子机器如何进化的有用模型。研究转录调控进化的一种方法是在远亲中测试来自一个物种的顺式元件的功能。以前的研究结果表明，很少，如果有的话，果蝇的组织特异性启动子在C。优雅在这里，我们表明，相反，苍蝇和人类的热休克基因的启动子上调C。elegans线虫on exposure暴露to heat热.热休克条件下的诱导可能代表一个相对简单的“开-关”反应，而复杂的表达模式需要多个信号的整合。我们的研究结果表明，较简单的方面的监管逻辑可能会保留在较长的进化时间，而更复杂的可能会更快地分化。
Transcriptional control of gene regulation is an intricate process that requires precise orchestration of a number of molecular components. Studying its evolution can serve as a useful model for understanding how complex molecular machines evolve. One way to investigate evolution of transcriptional regulation is to test the functions of cis-elements from one species in a distant relative. Previous results suggested that few, if any, tissue-specific promoters from Drosophila are faithfully expressed in C. elegans. Here we show that, in contrast, promoters of fly and human heat-shock genes are upregulated in C. elegans upon exposure to heat. Inducibility under conditions of heat shock may represent a relatively simple “on-off” response, whereas complex expression patterns require integration of multiple signals. Our results suggest that simpler aspects of regulatory logic may be retained over longer periods of evolutionary time, while more complex ones may be diverging more rapidly.