Different properties of two types of red fluorescent proteins in octocoral, Scleronephthya spp. as Akane families
Different properties of two types of red fluorescent proteins in octocoral, Scleronephthya spp. as Akane families
复制标题
八珊瑚、硬珊瑚属中两种红色荧光蛋白的不同特性。
DOI：
10.1002/bio.4394
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Toshio Yamaguchi
中科院分区：
文献类型：
--
作者：
Yuko Kato;Koji Yoshida;Yoshihito Ohba;Ikki Fujimoto;Yukimitsu Imahara;Shu Nakachi;Kenichiro Nakashima;Kosei Shioji;Toshio Yamaguchi
本文报道了从两种硬珊瑚（Scleronephthyasp）中提取的两种红色荧光蛋白（RFP）的不同性质。1（S. sp. 1）和S. sp，2（Alcyonacea，Nephtheidae）. sp. 1，命名为Alc-橙子，在449和574 nm激发下，分别在492 nm处发射强的绿色发射，在590和630 nm处发射弱的红色发射。sp. 2，LS-Red，当分别在574 nm和434 nm激发时，在642 nm发射强深红色，在480和510 nm发射弱绿色。当在434 nm处激发时，LS-Red具有约208 nm的非常大的斯托克斯位移，在642 nm处发射。有趣的是，LS-Red在pH 7.5下连续显示480（蓝色发射）、514（绿色发射）、563（橙子发射）和642 nm（深红色发射）的一些发射，这意味着在434 nm处一次激发产生多色荧光蛋白。在Alc-橙子的荧光的pH依赖性（pH 13 ~ 3.5）中，没有观察到“绿色和红色FP”之间的关系，而LS-Red显示出依赖于pH（11.5 ~ 4.5）的“绿色和红色形式”之间的相互转换。
We report the different properties of two types of red fluorescent proteins (RFP), undescribed species, extracted from two octocorals,Scleronephthyasp. 1 (S. sp. 1) andS. sp, 2 (Alcyonacea, Nephtheidae).S. sp. 1, named Alc‐Orange, emits strong green emission at 492 nm and weak red emission at 590 and 630 nm when excited at 449 and 574 nm, respectively.S. sp. 2, LS‐Red, emits strong deep red at 642 nm and weak green at 480 and 510 nm when excited at 574 nm and 434 nm, respectively. LS‐Red has a very large Stokes shift of about 208 nm emitting at 642 nm when excited at 434 nm. Interestingly, LS‐Red shows some emissions at 480 (blue emission), 514 (green emission), 563 (orange emission), and 642 nm (deep red emission) continuously at pH 7.5, which means multicolored fluorescence protein by one excitation at 434 nm. In pH dependence of fluorescence of Alc‐Orange (pH 13 to 3.5), no relation between ‘green and red FPs’ was observed, whereas LS‐Red showed the interconversion between ‘green and red forms’ depending on pH (11.5 to 4.5).