THE INTRACELLULAR GROWTH OF BACTERIOPHAGES .1. LIBERATION OF INTRACELLULAR BACTERIOPHAGE-T4 BY PREMATURE LYSIS WITH ANOTHER PHAGE OR WITH CYANIDE
THE INTRACELLULAR GROWTH OF BACTERIOPHAGES .1. LIBERATION OF INTRACELLULAR BACTERIOPHAGE-T4 BY PREMATURE LYSIS WITH ANOTHER PHAGE OR WITH CYANIDE
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DOI：
10.1085/jgp.35.4.645
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发表时间：
1952-01-01
影响因子：
3.8
通讯作者：
DOERMANN, AH
中科院分区：
文献类型：
--
作者：
DOERMANN, AH
本发明描述了一种用于在潜伏期的任何阶段释放和估计大肠杆菌中的细胞内噬菌体的方法。这是通过将感染的细菌置于含有0.01 [image]氰化物和高滴度T6裂解物的培养基中来实现的。在潜伏期的前半段，氰化物和T6都是必需的，但在第二天的半段，单独的氰化物将导致裂解和噬菌体释放。在潜伏期的前半段，细胞内未发现感染性噬菌体。在2d期间，半成熟噬菌体在细菌内以大约100%的速率积累。与细胞内DNA的增加呈线性平行，但滞后于DNA合成几分钟。使用5-甲基-异氰尿酸代替氰化物作为代谢抑制剂，发现了类似的结果，但是细胞内噬菌体曲线在时间轴上向左移动了几分钟。
A method is described for liberating and estimating intracellular bacteriophage in Escherichia coli at any stage during the latent period. This is brought about by placing the infected bacteria in a medium containing 0.01 [image] cyanide and a high titer T6 lysate. During the 1st half of the latent period both cyanide and T6 are necessary but in the 2d half cyanide alone will bring about lysis and phage liberation. During the 1st half of the latent period no infectious phage is found inside the cells. During the 2d half mature phage accumulates within the bacteria at a rate which is approx. linear and parallel to the increase in intracellular DNA, but lagging several min. behind DNA synthesis. Using 5-methyl-tryptophane in place of cyanide as the metabolic inhibitor similar results are found, but with the intracellular phage curves displaced to the left by several minutes on the time axis.