Novel procedure for high-fidelity tendon histology
Novel procedure for high-fidelity tendon histology
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DOI：
10.1002/jor.20304
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发表时间：
2007-03-01
影响因子：
2.8
通讯作者：
Wang, Vincent M.
中科院分区：
文献类型：
--
作者：
Laudier, Damien;Schaffler, Mitchell B.;Wang, Vincent M.
提出了一种获得高质量大鼠肌腱组织切片的新方法。这种方法利用无害的脱水和清洁溶剂和疏水丙烯酸（甲基丙烯酸甲酯）树脂。该方法避免了常规石蜡技术常见的处理和切片伪影，并克服了与亲水性（例如，乙二醇甲基丙烯酸酯）树脂组织学方法。这些新的组织学处理技术有助于可靠地评估肌腱的细胞和基质成分，并可以很容易地适应损伤，愈合和修复的形态学研究。(c)2006骨科研究学会。由威利期刊公司出版
A novel method to obtain high-quality histologic sections of rat tendon for light microscopic study is presented. This approach utilizes non-deleterious dehydrating and clearing solvents and a hydrophobic acrylic (methyl methacrylate) resin. This methodology avoids processing and microtomy artifacts common with routine paraffin wax techniques and overcomes specimen size limitations associated with hydrophilic (e.g., glycol methacrylate) resin histologic methods. These novel histologic processing techniques facilitate the reliable assessment of tendons' cellular and matrix components and can be readily adapted to morphologic studies of damage, healing, and repair. (c) 2006 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.