Excitatory effects of thyrotropin-releasing hormone on neurons within the nucleus ambiguus of adult guinea pigs.
Excitatory effects of thyrotropin-releasing hormone on neurons within the nucleus ambiguus of adult guinea pigs.
复制标题
促甲状腺素释放激素对成年豚鼠疑核内神经元的兴奋作用。
DOI：
10.1016/0006-8993(92)91074-o
复制
发表时间：
1992
期刊：
影响因子：
2.9
通讯作者：
Getting,PA
中科院分区：
文献类型：
--
作者：
Johnson,SM;Getting,PA
用离体豚鼠脑干脑片研究了促甲状腺素释放激素（TRH）对疑核（NA）神经元的电生理作用。在0.01-1.0 μ mTRH中，NA神经元出现去极化（25/39）、抑制后反弹增强（8/8）或膜电位振荡（17/39）。由于TRH不改变河豚毒素（TTX）在不同膜电位下的抑制后反弹幅度，这表明TRH通过去极化细胞膜间接增强抑制后反弹。NA神经元的膜电位振荡是持久的TTX和它们的频率依赖于膜电位，这表明这些振荡是由于内在的膜特性，而不是突触输入。TRH对离体NA神经元的兴奋作用表明，内源性TRH可调节参与呼吸和自主神经功能调节的神经元的活动。
The electrophysiological effects of thyrotropin-releasing hormone (TRH) on neurons within the nucleus ambiguus (NA) of adult guinea pigs were studied using an in vitro brain stem slice preparation. In 0.01–1.0 μm TRH, NA neurons depolarized (25/39), expressed enhanced postinhibitory rebound (8/8 tested), or exhibited oscillations of the membrane potential (17/39). Because the amplitude of postinhibitory rebound in tetrodotoxin (TTX) at various membrane potentials was not altered by TRH, it suggests that TRH enhanced postinhibitory rebound indirectly by depolarizing the cell membrane. The membrane potential oscillations in NA neurons were persistent in TTX and their frequency was dependent on the membrane potential, suggesting that these oscillations were due to intrinsic membrane properties and not to synaptic inputs. The excitation of NA neurons in vitro by TRH suggests that endogenous TRH may modulate the activity of neurons involved in the regulation of respiratory and autonomic function.