Effects of norepinephrine infusion on myocardial high-energy phosphate content and turnover in the living rat.
Effects of norepinephrine infusion on myocardial high-energy phosphate content and turnover in the living rat.
复制标题
去甲肾上腺素输注对活体大鼠心肌高能磷酸盐含量和周转的影响。
DOI：
10.1172/jci113027
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Ingwall,JS
中科院分区：
文献类型：
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作者：
Bittl,JA;Balschi,JA;Ingwall,JS
应用~（31）P-核磁共振技术，研究了去甲肾上腺素输注和氟烷麻醉下，大鼠心肌高能磷酸盐含量与肌酸激酶反应通量之间的关系。在2%氟烷麻醉（n = 4）、1%氟烷麻醉（n = 5）和去甲肾上腺素输注（n = 4）下，大鼠分别产生19.5 +/- 1.6、32.0 +/- 3.5和48.5 +/- 2.0 × 1，000 mmHg/min的心率-血压乘积。三磷酸腺苷含量不受变力状态的影响，范围为24.3 +/- 1.1至25.6 +/- 1.1 mumol/g干重，但磷酸肌酸含量与心脏性能呈负相关且可逆变化，从2%氟烷下的45.6 +/- 6.0变化至去甲肾上腺素输注期间的26.0 +/- 6.5 mumol/g干重。在2%氟烷、1%氟烷和含去甲肾上腺素的1%氟烷下，肌酸激酶反应的通量值分别为15.4 +/- 4.6、20.5 +/- 2.0和30.1 +/- 7.9 mumol/g干重/s。这些结果表明，心肌高能磷酸盐化合物的周转，而不是其组织内容物，在变力性刺激期间与心脏性能相匹配。图片
Using 31P-nuclear magnetic resonance, we studied the relationship between myocardial high-energy phosphate content and flux values for the creatine kinase reaction in the living rat under inotropic states achieved during norepinephrine infusion and halothane anesthesia. Under 2% halothane anesthesia (n = 4), 1% halothane anesthesia (n = 5) and norepinephrine infusion (n = 4), rats developed rate-pressure products of 19.5 +/- 1.6, 32.0 +/- 3.5, and 48.5 +/- 2.0 X 1,000 mmHg/min, respectively. Adenosine triphosphate content was not affected by inotropic state, ranging from 24.3 +/- 1.1 to 25.6 +/- 1.1 mumol/g dry weight, but creatine phosphate content varied inversely and reversibly with cardiac performance from 45.6 +/- 6.0 under 2% halothane to 26.0 +/- 6.5 mumol/g dry weight during norepinephrine infusion. The flux values for the creatine kinase reaction were 15.4 +/- 4.6, 20.5 +/- 2.0, and 30.1 +/- 7.9 mumol/g dry weight per s under 2% halothane, 1% halothane, and 1% halothane with norepinephrine, respectively. These results suggest that the turnover of myocardial high-energy phosphate compounds, not their tissue contents, matches cardiac performance during inotropic stimulation.Images