Collapsin Response Mediator Protein 1 Mediates Reelin Signaling in Cortical Neuronal Migration
Collapsin Response Mediator Protein 1 Mediates Reelin Signaling in Cortical Neuronal Migration
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DOI：
10.1523/jneurosci.4276-06.2006
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发表时间：
2006-12
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通讯作者：
N. Yamashita;Yutaka Uchida;T. Ohshima;S. Hirai;F. Nakamura;M. Taniguchi;K. Mikoshiba;J. Honnorat;P. Kolattukudy;N. Thomasset;K. Takei;Takuya Takahashi;Y. Goshima
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作者：
N. Yamashita;Yutaka Uchida;T. Ohshima;S. Hirai;F. Nakamura;M. Taniguchi;K. Mikoshiba;J. Honnorat;P. Kolattukudy;N. Thomasset;K. Takei;Takuya Takahashi;Y. Goshima
塌陷反应介质蛋白1 （Collapsin response mediator protein 1, CRMP1）是介导轴突引导分子信号转导的CRMP家族成员之一。在这里，我们展示了CRMP1参与Reelin （Reln）信号传导调节大脑皮层的神经元迁移的证据。在crmp1−/−小鼠中，皮层神经元的径向迁移被延缓。在sema3A−/−和crmp1+/−中未观察到这种表型；sema3A + /−皮质。然而，CRMP1与禁用-1 （Dab1）共定位，后者是Reln信号传导的一种衔接蛋白。在Relnrl/rl皮层中，CRMP1和Dab1的表达水平较高，而酪氨酸的磷酸化水平较低。在dab1杂合背景下，crmp1的缺失导致海马层压的破坏，这是一种reeler样表型。除了轴突引导外，CRMP1还通过介导Reln信号传导调节神经元迁移。
Collapsin response mediator protein 1 (CRMP1) is one of the CRMP family members that mediates signal transduction of axon guidance molecules. Here, we show evidence that CRMP1 is involved in Reelin (Reln) signaling to regulate neuronal migration in the cerebral cortex. In crmp1−/− mice, radial migration of cortical neurons was retarded. This phenotype was not observed in the sema3A−/− and crmp1+/−;sema3A+/− cortices. However, CRMP1 was colocalized with disabled-1 (Dab1), an adaptor protein in Reln signaling. In the Relnrl/rl cortex, CRMP1 and Dab1 were expressed at a higher level, yet tyrosine phosphorylated at a lower level. Loss of crmp1 in a dab1 heterozygous background led to the disruption of hippocampal lamination, a Reeler-like phenotype. In addition to axon guidance, CRMP1 regulates neuronal migration by mediating Reln signaling.