Dilute sulfuric acid pretreatment of corn stover for enzymatic hydrolysis and efficient ethanol production by recombinant Escherichia coli FBR5 without detoxification
Dilute sulfuric acid pretreatment of corn stover for enzymatic hydrolysis and efficient ethanol production by recombinant Escherichia coli FBR5 without detoxification
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DOI：
10.1016/j.biortech.2013.05.002
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发表时间：
2013-08-01
影响因子：
11.4
通讯作者：
Cotta, Michael A.
中科院分区：
文献类型：
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作者：
Avci, Ayse;Saha, Badal C.;Cotta, Michael A.
为了减少预处理过程中抑制物质的产生，使发酵前不需要脱毒步骤，同时最大限度地提高糖产量，开发了一种用于稀硫酸预处理玉米秸秆的预处理策略。玉米秸秆（10%，w/v）预处理的最佳条件为：0.75%H_2SO_4，160 ℃，0-5 min。基于酶促水解后的最大葡萄糖释放、戊糖的最小损失和糖降解产物如糠醛和羟甲基糠醛的最小形成来选择条件。酶糖化后的预处理玉米秸秆在0和5分钟保持时间下分别产生63.2 +/-2.2和63.7 +/-2.3g总糖/L。糠醛产量分别为0.45 +/- 0.1和0.87 +/- 0.4 g/L。重组大肠杆菌菌株FBR 5有效发酵未脱毒玉米秸秆水解液，如果糠醛含量为
A pretreatment strategy for dilute H2SO4 pretreatment of corn stover was developed for the purpose of reducing the generation of inhibitory substances during pretreatment so that a detoxification step is not required prior to fermentation while maximizing sugar yield. The optimal conditions for pretreatment of corn stover (10%, w/v) were: 0.75% H2SO4, 160 degrees C, and 0-5 min holding time. The conditions were chosen based on maximum glucose release after enzymatic hydrolysis, minimum loss of pentose sugars and minimum formation of sugar degradation products such as furfural and hydroxymethyl furfural. The pretreated corn stover after enzymatic saccharification generated 63.2 +/- 2.2 and 63.7 +/- 2.3 g total sugars per L at 0 and 5 min holding time, respectively. Furfural production was 0.45 +/- 0.1 and 0.87 +/- 0.4 g/L, respectively. The recombinant Escherichia coli strain FBR5 efficiently fermented non-detoxified corn stover hydrolyzate if the furfural content is