Meningococcal carriage rates in healthy individuals in Japan determined using Loop-Mediated Isothermal Amplification and oral throat wash specimens
Meningococcal carriage rates in healthy individuals in Japan determined using Loop-Mediated Isothermal Amplification and oral throat wash specimens
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DOI：
10.1016/j.jiac.2015.12.016
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发表时间：
2016-07-01
影响因子：
2.2
通讯作者：
Ohnishi, Makoto
中科院分区：
文献类型：
--
作者：
Takahashi, Hideyuki;Haga, Masae;Ohnishi, Makoto
日本脑膜炎双球菌疾病的详细流行病学尚未确定，此外，健康携带率也未知。为了了解日本健康人群中脑膜炎奈瑟菌的带菌率，我们建立了一种新的检测脑膜炎奈瑟菌的ctrB基因的方法，该基因是编码脑膜炎奈瑟菌包膜合成的酶基因之一。LAMP检测结果显示，836份标本中有7份脑膜炎奈瑟菌DNA阳性，并用脑膜炎球菌特异性GGT基因的套式聚合酶链式反应方法进行了验证。据估计，健康人群的脑膜炎奈瑟菌携带率为0.84%。进一步通过套式-聚合酶链式反应的血清分型方法进一步证实，5份阳性标本属于Y群，1份属于B群，1份无法鉴定。考虑到日本脑膜炎球菌病的流行病学，带菌率和血清群分布似乎分别与日本脑膜炎球菌病的低发病率和临床脑膜炎球菌分离株的血清群分布相一致。(C)2016年日本化疗学会和日本传染病协会。爱思唯尔有限公司出版。保留所有权利。
The detailed epidemiology of meningococcal diseases in Japan has yet to be determined and, moreover, the healthy carriage rate is also unknown. In this study, to obtain insight into the carriage rate of Neisseria meningitidis in healthy individuals in Japan, we developed a new method to detect the N. meningitidisspecific ctrB gene, one of the genes encoding enzymes for capsule synthesis, by Loop-Mediated Isothermal Amplification (LAMP) and examined the meningococcal carriage rate by using self collected oral throat wash specimens from 836 students at a university. Examination by LAMP showed that 7 out of 836 samples were positive for N. meningitidis DNA, and the results were also verified by the nested PCR method for the meningococcus specific ggt gene. The N. meningitidis carriage rate in healthy individuals was estimated to be 0.84%. Moreover, we further confirmed by the nested-PCR-based serogroup typing method that 5 of the positive samples belonged to serogroup Y, 1 belonged to group B and 1 was unidentifiable. Considering the epidemiology for meningococcal diseases in Japan, the carriage rate and the serogroup profile seem to be consistent with low incidence of meningococcal diseases and serogroup distribution of clinical meningococcal isolates in Japan, respectively. (C) 2016 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.