Ectopic osteogenesis by type I collagen loaded with a novel synthesized PTH-related peptide-1 in vivo
Ectopic osteogenesis by type I collagen loaded with a novel synthesized PTH-related peptide-1 in vivo
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体内负载新型合成 PTH 相关肽-1 的 I 型胶原异位成骨
DOI：
10.1002/jbm.a.36802
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发表时间：
2020-01-01
影响因子：
4.9
通讯作者：
Li, Jingfeng
中科院分区：
文献类型：
--
作者：
Wang, Yi;Wang, Jianping;Li, Jingfeng
本研究旨在探讨甲状旁腺激素（PTH）相关肽-1/I型胶原（PTHrP-1/Col-I）支架材料诱导SD大鼠股四头肌袋异位成骨的能力。一种新的肽PTHrP-1来自PTH，并以不同的浓度（0，0.1，0.3和0.5 mg/ml）诱导异位成骨。影像学检查（X线、CT、三维重建）、病理学观察（H&E、Masson、Von Kossa、ALP、TRAP染色）、免疫组化染色（组织中Col-I、OCN、Runx-2）、Western blotting等方法对相关标志物蛋白进行定性和定量分析。结果证实，适当浓度的PTHrP-1能有效增强SD大鼠股四头肌肌袋的成骨活性，从而提高成骨标志物（COL-I、OCN和Runx-2）的阳性结果和蛋白表达。通过定性和定量分析，高浓度的PTHrP-1促进成骨活性和活跃的骨吸收。本研究证实PTHrP-1是一种新型的小分子生物活性肽，鼠尾胶原支架是PTHrP-1的良好载体，具有良好的生物相容性。PTHrP-1/Col-I复合支架材料是一种有效的骨组织工程替代物，能有效诱导和促进大鼠股四头肌肌袋内骨形成。此外，0.3 mg/ml PTHrP-1/Col-I复合支架材料组的成骨分化促进能力明显优于0、0.1和0.5 mg/ml PTHrP-1/Col-I复合支架材料组。因此，PTHrP-1促进异位成骨的最佳浓度为0.3 mg/ml。
This study aims to investigate the ability of parathyroid hormone (PTH)-related peptide-1/type I collagen (PTHrP-1/Col-I) scaffold material to induce ectopic osteogenesis in the quadriceps muscle pocket of Sprague-Dawley (SD) rats. A novel peptide PTHrP-1 was derived from PTH and used at different concentrations (0, 0.1, 0.3, and 0.5 mg/ml) to induce ectopic osteogenesis. Radiographic examinations (X-ray, CT, and 3D reconstruction), pathological observations (H&E, Masson, Von Kossa, ALP and TRAP staining), immunohistochemical staining (Col-I, OCN and Runx-2 in tissues), western blotting was used to determine the qualitative and quantitative analysis of related markers proteins. Results confirmed that the appropriate concentration of PTHrP-1 can effectively enhance the osteogenic activity, thereby improving the positive results and protein expression of osteogenic markers (COL-I, OCN, and Runx-2) in the quadriceps muscle pocket of SD rats. Through qualitative and quantitative analysis, high concentration of PTHrP-1 promotes osteogenic activity and active bone resorption. This study confirmed that PTHrP-1 is a novel small molecule bioactive peptide, and the rat tail collagen scaffold is a good carrier of PTHrP-1 with excellent biocompatibility. The PTHrP-1/Col-I composite scaffold material is an effective substitute for bone tissue engineering and can effectively induce and promote bone formation in the quadriceps muscle pocket of rats. In addition, the promoting ability for osteogenic differentiation of 0.3 mg/ml PTHrP-1/Col-I composite scaffold material group was significantly better than that of 0, 0.1 and 0.5 mg/ml PTHrP-1/Col-I composite scaffold material groups. Hence, the optimal concentration of PTHrP-1 to promote ectopic osteogenesis is 0.3 mg/ml.