Breakthrough in Marine Invertebrate Cell Culture: Sponge Cells Divide Rapidly in Improved Nutrient Medium
Breakthrough in Marine Invertebrate Cell Culture: Sponge Cells Divide Rapidly in Improved Nutrient Medium
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DOI：
10.1038/s41598-019-53643-y
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发表时间：
2019-11-21
影响因子：
4.6
通讯作者：
Pomponi, Shirley A.
中科院分区：
文献类型：
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作者：
Conkling, Megan;Hesp, Kylie;Pomponi, Shirley A.
海绵（门Porifera）是最古老的后生动物之一，被认为是理解动物进化和发展的关键。它们也是与制药有关的海洋衍生化学品的最多产来源。细胞系是许多学科研究的重要工具，并已在许多生物中建立，包括淡水和陆生无脊椎动物。尽管经过了几十年的努力，仍然没有海洋无脊椎动物的细胞系。在这项研究中，我们报告了一项突破：我们证明了氨基酸优化的营养培养基可以刺激9种海绵物种的快速细胞分裂。分裂最快的细胞在不到一小时内翻了一番。3种培养基传代3 ~ 5次，传代后平均翻倍5.99倍，寿命21 ~ 35天。我们的研究结果为开发海洋无脊椎动物细胞模型奠定了基础，以更好地了解动物早期进化，确定次生代谢物的作用，并预测气候变化对珊瑚礁群落生态的影响。此外，海绵细胞系可用于扩大海绵衍生化学品的生产，用于临床试验，并开发对抗癌症和其他疾病的新药。
Sponges (Phylum Porifera) are among the oldest Metazoa and considered critical to understanding animal evolution and development. They are also the most prolific source of marine-derived chemicals with pharmaceutical relevance. Cell lines are important tools for research in many disciplines, and have been established for many organisms, including freshwater and terrestrial invertebrates. Despite many efforts over multiple decades, there are still no cell lines for marine invertebrates. In this study, we report a breakthrough: we demonstrate that an amino acid-optimized nutrient medium stimulates rapid cell division in 9 sponge species. The fastest dividing cells doubled in less than 1 hour. Cultures of 3 species were subcultured from 3 to 5 times, with an average of 5.99 population doublings after subculturing, and a lifespan from 21 to 35 days. Our results form the basis for developing marine invertebrate cell models to better understand early animal evolution, determine the role of secondary metabolites, and predict the impact of climate change to coral reef community ecology. Furthermore, sponge cell lines can be used to scale-up production of sponge-derived chemicals for clinical trials and develop new drugs to combat cancer and other diseases.